Purpose: The objective of this study is to determine the genotypic variations of the antimicrobial resistance profile among Salmonella isolates from farm/field to fork (i.e., animal, food and human) collected in Turkey, 2012.
Methods: Strains were gathered from southeast and median Anatolian region of Turkey. The isolates were from veterinary, human and food. For Salmonella isolation, ISO6579 procedure was used. The confirmation of Salmonella isolates was done by invA gene on PCR. Serotyping of Salmonella was performed according to the Kauffman-White Procedure. Phenotypic antimicrobial resistance typing was done by disk diffusion method. 19 different antimicrobial elements were used. 21 antimicrobial resistance genes were searched in 90 phenotypically resistant Salmonella isolates.
Results: 74 food-related, 54 animal-related and 50 clinical human Salmonella isolates were investigated. 26 different serotypes were determined. Chicken isolates take the attention since all were found to be resistant at least one antimicrobial agent, and most of them belong to serotype S. Infantis (90.5%). Every Infantis isolate was resistant to nalidixic acid and tetracycline. Differently from food isolates, animal isolates were observed to have high rate of resistance against beta-lactam groups. Antimicrobial resistance profiles of Salmonella Hadar isolates that were obtained from food and animal samples were similar.
Significance: Our study fills the gap of limited relevant studies about the antibiotic susceptibility profile of Salmonella isolates from farm/field to fork in Turkey. Our study has the potential of being a progressive work conducted in the pathogenicity area.