Back to Basics: What is Whole Genome Sequencing? Why the Hype?

The ability to rapidly generate and analyze whole genome sequence data is here. The advent of tabletop next generation sequencers allows for routine sequencing of microbial isolates, entire populations and even complex samples such as clinical specimens and food and other environmental samples. Genome analysis allows for the careful analysis of all genetic features and the precise understanding of relationships between and among isolates and populations. Such power is rapidly transforming both the clinical and public health response to food-borne and other infectious disease outbreaks. An overview of the current sequence and analysis technology will be provided as well as multiple approaches to how these technologies can be used to detect pathogens, solve outbreaks, conduct disease surveillance and better understand emerging pathogens.