Purpose: In this study, the fate of DON and ZEN during milling of Korean rice cultivars was investigated.
Methods: Rice (n = 80) grown under different field production conditions were collected and milled using a laboratory-scale test mill to produce four fractions: white rice, bran, brown rice and hulls. The four milling fractions were analyzed for DON and ZEN with a validated analytical method using high-performance liquid chromatography (HPLC) with UV absorbance and fluorescence detection.
Results: The contamination levels in white rice, bran, brown rice and hulls were 0, 0.5, 4.1 and 1.9 µg/kg for DON, and 0.6, 33.0, 156.6 and 923.1 µg/kg for ZEN, respectively. Among 320 milling fractions, the incidence of DON was ranged from 0 to 8.8%. In contrast, ZEN was detected in all milling fractions, but exponentially reduced through milling process.
Significance: Understanding the effect of milling that impact on the fractionation of DON and ZEN milling will help rice processors and/or risk managers to control rice-based products within legislative limits.