Purpose: To investigate the effect of pulsed light on the survival of a predetermined population of Salmonella Enteritidis(PT30), spot inoculated on the surface of almonds.
Methods: Spot-inoculated samples were exposed to pulsed light at a rate of 3 pulses/sec, for 10, 30 and 60 sec at a distance of 14.1, 16.6, and 19.2 cm from the central axis of the lamp. Samples were also intermittently treated for 60 sec with 10-sec treatment followed by a 3-min cooling period. The surviving population was enumerated.
Results: A maximum inactivation of 3.36 ± 0.19 log CFU/almond was obtained after a 30 sec exposure to pulsed light at a distance of 14.1 cm away from the lamp axis. Even a 10 sec treatment resulted in 2.88 ± 0.21 log CFU/almond reduction at 14.1 cm from the lamp axis. As expected, microbial inactivation decreased as the distance increased with reductions of 3.36 ± 0.19, 1.76 ± 0.09, 1.25 ± 0.04, and log CFU/almond were obtained at 14.1, 16.6 and 19.2 cm, respectively. Intermittent treatment of samples (10-sec treatment followed by 3-min cooling for 60 sec) resulted in up to 1.02-log CFU/almond increase in the microbial reduction depending upon the distance from the lamp housing.
Significance: Results clearly indicate that pulsed light is effective as a surface decontamination technology for inactivation of Salmonella on almond surfaces.