Purpose: The aim of this study is to determine the influence of water mobility on the survival of Salmonellain low moisture whey protein powder held at 80°C.
Methods: Whey protein powder (95% protein) of differing water mobilities was produced by pH adjustment (2, 5, and 7), heat denaturation and equilibration to various aw levels between 0.18 and 0.52. Powders were inoculated with a four-strain cocktail of Salmonella, vacuum-sealed and immersed in a circulating water bath at 80 °C for 48 hr. Cells were recovered at various times on nonselective differential media. Microbial survival data were analyzed using ANOVA.
Results: Water activity significantly influences the heat inactivation of Salmonella (P < 0.0001). Treatment after 4 hours showed average log reduction values of 2.7, 4.6, 5.0, 5.2 and 5.1 at aw levels of 0.18, 0.29, 0.36, 0.42 and 0.52, respectively. Water mobility had a significant effect on microbial death (P = 0.030) at higher aw levels (0.36 to 0.52), with average log reductions of 4.8 and 5.4 at lower and higher mobilities, respectively. Water mobility did not significantly influence inactivation in the aw range of 0.18 to 0.29 (P = 0.598).
Significance: Water mobility influences the survival of Salmonella spp. in low moisture protein powder at aw levels between 0.36 and 0.52 during treatment at 80 °C.