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P1-153 Identification of Fish Samples through DNA Barcoding: A Surveillance Study of Public Health Importance

Monday, July 29, 2013
Exhibit Hall (Charlotte Convention Center)
Irshad Sulaiman, U.S. Food and Drug Administration, Atlanta, GA
Emily Jacobs, U.S. Food and Drug Administration, Atlanta, GA
Nancy Miranda, U.S. Food and Drug Administration, Atlanta, GA
Steven Simpson, U.S. Food and Drug Administration, Atlanta, GA
Khalil Kerdahi, U.S. Food and Drug Administration, Atlanta, GA
Introduction: The DNA barcoding has been widely successful in species identification in a wide range of zoonotic species, including fish.  It uses the 5’ region of the mitochondrial cytochrome oxidase I (COI) gene as the genetic marker. The primary mission of FDA is to warrant a safe food supply chain and correctly label food and food products. This task is indeed very challenging as over 80% of seafood consumed in the United States is imported, and there are over 28, 000 known species of fish.

Purpose: The major objective of this study was to evaluate and validate the DNA barcoding method that can be used in our laboratory, for rapid species identification of fish samples from routine surveillance, foodborne outbreak, and in species substitution and economic fraud investigations.  

Methods:  In this study, we standardized the protocols for DNA extraction, and COI gene based PCR amplification and bi-directional nucleotide sequencing of the standard barcode region (655 bp).  Initially, we carried out a validation study using 32 samples belonging to 4 known fish species. Later, we successfully performed the DNA barcoding for 64 surveillance import samples of 12 fish species collected from Argentina, Brazil, China, Norway, United Kingdom, and Vietnam. Data was analyzed using the GENEIOUS program. The analytical tools on BOLD and GenBank were also utilized to assess the performance of barcoding to identify species.

Results: High quality bases (>98%HQ-100%HQ) were obtained for all the samples sequenced. Using neighbor-joining distance comparison, the genetic distance was measured and the phylogenetic tree was constructed. The generated COI nucleotide sequences provided a complete species-identification to the fish samples analyzed.

Significance: The results suggest that DNA barcoding is a very effective method for species identification of fishes. This will further help in accomplishing the mission of our agency.

Back to: Poster Session 1 - Risk Assessment, Food Toxicology, Beverages & Water, Meat & Poultry, Dairy & Other Food Commodities, Food Defense, Communication Outreach & Education, Non-microbial Food Safety, General Microbiology, Epidemiology, Seafood, Microbial Foo
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