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P1-123 Role of Egg-yolk Antibody (EYA) in Protection against Bacterial Association in Salmonella-poultry Cell Interactions

Monday, July 29, 2013
Exhibit Hall (Charlotte Convention Center)
Jose Alejandro Almario, University of Maryland-College Park, College Park, MD
Serajus Salaheen, University of Maryland-College Park, College Park, MD
Juhee Ahn, Kangwon National University, Chuncheon, South Korea
Daniel Hewes, University of Maryland-College Park, College Park, MD
Debabrata Biswas, University of Maryland-College Park, College Park, MD
Introduction:  Salmonella enterica serovars Enteritidis (SE) and Typhimurium (ST) are the most common serovars isolated from egg and poultry products. To meet the growing organic poultry sector and to address antibiotic resistance in conventional poultry, alternative antimicrobial strategies are essential. Works demonstrate egg-yolk antibodies (EYA) immunogenic properties against enteric pathogens but their role in decreasing colonization of SE and ST in poultry has not been demonstrated clearly.

Purpose:  The purpose of this study was to investigate the ability of the water-soluble fraction (WSF) of egg-yolks containing EYAs to decrease the association of SE and ST to chicken cells culture model.

Methods:  A chicken fibroblast, DF-1, and chicken macrophage-like cell line, HD-11, were used in this study. Semiconfluent monolayers of cells were treated with four different WSFs at various concentrations. Salmonella suspensions were added for infection. Monolayers were washed and associated bacteria were enumerated after serial dilution.

Results:  Western blot analysis revealed non-specific binding of the WSF with bacterial cell lyses of ST and SE. Association of ST in DF-1depended on the application of WSF. For example in DF-1 cells, untreated log CFU were 5.86 ± 0.02 vs. 4.5 ± 0.33 (P < 0.0001). In HD-11, application of the various WSF increased and decreased association, depending on WSF applied; untreated log CFU were 3.86 ± 0.22 vs. 3.05 ± 0.03 vs. 4.52 ± 0.16 (P < 0.0001). Reduction was also nuanced in SE infection; in DF-1 cells, untreated log CFU were 5.60 ± 0.01 vs. 4.03 ± 0.20 (P < 0.0001). Similar HD-11 results were also found, where untreated log CFU were 3.86 ± 0.22 vs. 2.97 ± 0.09 vs. 4.38 ± 0.09 (P < 0.0001, P = .0011)

Significance:  These findings demonstrate the need for research identifying necessary adhesins for Salmonella infection in chickens. While EYA application may be viable, development of encapsulation methods may enhance efficacy of the treatment.

Back to: Poster Session 1 - Risk Assessment, Food Toxicology, Beverages & Water, Meat & Poultry, Dairy & Other Food Commodities, Food Defense, Communication Outreach & Education, Non-microbial Food Safety, General Microbiology, Epidemiology, Seafood, Microbial Foo
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