Purpose: Compare the outgrowth of Clostridium perfringensduring a 15 h cooling curve in turkey breast cured with nitrite from purified and natural sources.
Methods: Five treatments of deli-style turkey breast (74% moisture, pH 6.2-6.4, 1.4% NaCl) were prepared: control (0 NaNO2), 100 ppm NaNO2 from both purified nitrite and pre-converted celery juice powder, 100 ppm purified nitrite + 547 ppm ascorbate, and 100 ppm pre-converted nitrite + 547 ppm ascorbate from cherry powder. Treatments were inoculated with C. perfringens spores (three-strain mixture) to yield 3 log CFU/g. Individual 50-g portions were vacuum-packaged, cooked to 71.1°C and cooled from 54.4°C to 26.7°C in 5 hours and 26.7°C to 7.2°C in 10 additional hours. Triplicate samples were assayed for growth of C. perfringens at 0, 2.5, 5, 7.5, 10, 12.5, and 15 h by plating on tryptose-sulfite-cycloserine agar; experiments were replicated three times.
Results: Control, purified nitrite, and pre-converted nitrite treatments showed > 1 log increase after 5 h and 5.3±0.2, 4.2±0.6, and 4.4±0.2 log increases at 15 h, respectively, revealing no difference in growth inhibition between the two nitrite sources and only slight inhibition by 100 ppm NaNO2 relative to control. In contrast, < 1 log growth was observed through 15 h in treatments containing 100 ppm nitrite and 547 ppm ascorbate.
Significance: This study confirmed that equivalent concentrations of nitrite, regardless of the source, provide similar inhibition of C. perfringens during cooling and suggests greater inhibition exists when combined with a cure accelerator.