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P1-139 Development of Epidemiological Investigation System Using Mobile Lab in Korea

Monday, July 29, 2013
Exhibit Hall (Charlotte Convention Center)
Soo-Il Ko, Korea Food and Drug Administration, Chungcheongbuk-do, South Korea
Joon Il Cho, Korea Food and Drug Administration, Chungcheongbuk-do, South Korea
Kun Sang Park, Korea Food and Drug Administration, Chungcheongbuk-do, South Korea
Soo Bok Kim, Kogenebiotech Co., Ltd., Seoul, South Korea
Yong Suk Nam, Kogenebiotech Co., Ltd., Chungcheongbuk-do, South Korea
Hyung Joo Yoon, Korea Food and Drug Administration, Chungcheongbuk-do, South Korea
Hyo Sun Kwak, Korea Food and Drug Administration, Chungcheongbuk-do, South Korea
Introduction: Effective control of foodborne outbreaks is very important to public health, and it is necessary for early detection and identification of foodborne pathogens and immediate removal of the suspected food from the market, accompanied by preventing secondary spread.

Purpose: To ensure preventing foodborne disease and operating early warning system, KFDA established the mobile laboratory which is designed for on-site testing of 16 foodborne pathogens.

Methods: The mobile laboratory is equipped with the instruments including a DNA extraction system, Real-time PCR and reagents for DNA assay. In order to analyze foodborne pathogens in foods and feces, the PowerCheKTM 19 Pathogen Multiplex Real-time PCR Kit (developed by KogeneBiotech Co., Ltd.) has been used. The assay was carried out to detect and identify 19 specific genes of 16 pathogens, simultaneously, by using multiplex primer and probe system labeled with different fluorephores.

Results: The KFDA Mobile Lab has successfully performed on-site testing during the "2011 World Nuclear Summit" and the "Yeosu EXPO 2012" etc. A total 838 food samples were tested and food pathogens such as Vibrio parahaemolyticus and Listeria monocytogeneswere detected from 11 samples. Also, it has confirmed the cause of outbreak occurring in 8 school caterings immediately and prevented the possible spread of additional foodborne outbreak in August, 2012.

Significance: The KFDA Mobile Lab can simplify on-site food testing and be applied to efficient monitoring of the outbreak of foodborne pathogen. These applications will provide early warning against the outbreak and assist food safety and regulatory system for the compliance of the relevant rules and regulations.

Back to: Poster Session 1 - Risk Assessment, Food Toxicology, Beverages & Water, Meat & Poultry, Dairy & Other Food Commodities, Food Defense, Communication Outreach & Education, Non-microbial Food Safety, General Microbiology, Epidemiology, Seafood, Microbial Foo
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