P3-124 Validation of the New Rapid Detection System - 3M Petrifilm Salmonella Express System for the Detection of Salmonella in Raw and Processed Seafood Products

Wednesday, August 6, 2014
Exhibit Hall D (Indiana Convention Center)
Kitiya Vongkamjan, Prince of Songkla University, Hat Yai, Thailand
Janejira Fuangpaiboon, 3M Food Safety, 3M Thailand Ltd., Bangkok, Thailand
Bussara Sripaurya, Prince of Songkla University, Hat Yai, Thailand
Matthew Turner, 3M Asia Pacific, Singapore, Singapore
Introduction: Seafood products are a major food category for Thailand’s export. Salmonella contamination in seafood products has been frequently reported, raising a global public health concern. A new rapid detection system, 3M™ Petrifilm™ Salmonella Express (SALX) System, employs a simpler all-in-one method (sample enrichment to confirmation of results) for screening of Salmonella in food products, and yields faster and reliable results.

Purpose: This study evaluated the performance of the system for the detection of Salmonella in a variety of raw and processed seafood products from Thailand as compared to the standard ISO 6579-1:2007 method.

Methods: Products (n = 200) were purchased, as manufacturer-sealed packaging or individually weighed/sliced from retail counters, markets, and street vendors. Products included 43 raw seafood products, 66 processed (ready-to-cook) seafood products (e.g., dried assorted fish), and 91 RTE products (e.g., mixed fish wonton). Each sample tested (approx. 100 g) was homogenized and screened for Salmonella following two methods; the system and ISO 6579-1:2007.

Results: The system showed comparable detection results to those obtained from the ISO method. Overall, 112/200 samples were positive for Salmonella (56%). Twelve samples tested positive by the system only. These samples encompassed a variety of different food matrices; six were products with high microbial loads. The sensitivity of the system was 100% (95% CI: 95.4-100%), specificity was 88.0% (95% CI: 81.6-94.4%), accuracy was 94.0%, and the positive predictive value was 89.3% (95% CI: 83.6-95.0%).

Significance: Rapid detection and monitoring of Salmonella in seafood products can improve seafood safety and quality in domestic and international markets. The 3M Petrifilm SALX System is a new all-in-one detection method that can facilitate high-throughput sample screening for Salmonella to obtain faster and reliable results.