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P2-66 Performance Evaluation of DuPont™ BAX® System Real-time PCR Assay for Salmonella Enteritidis and Typhimurium

Tuesday, August 5, 2014
Exhibit Hall D (Indiana Convention Center)
Jun Li, DuPont Nutrition & Health, Wilmington, DE
Mark Jensen, DuPont Nutrition & Health, Wilmington, DE
Angeline Stoltzfus, DuPont Nutrition & Health, Wilmington, DE
Tan Ling, China National Accreditation Service, Beijing, China
Dawn Fallon, DuPont Nutrition & Health, Wilmington, DE
Krystal Shortlidge, DuPont Nutrition & Health, Wilmington, DE
Eugene Davis, DuPont Nutrition & Health, Wilmington, DE
Jeffrey Rohrbeck, DuPont Nutrition & Health, Wilmington, DE
Emily Tay, DuPont Nutrition & Health, Singapore, Singapore
Stephen Varkey, DuPont Nutrition & Health, Wilmington, DE
Daniel DeMarco, DuPont Nutrition & Health, Wilmington, DE
Introduction: Salmonella Enteritidis and Typhimurium are among the top serovars significant in public health. In response to demands for a faster, simpler method for identifying these organisms, A novel Assay was developed for Salmonella Enteritidis and Typhimurium (including its monophasic variant, 4,5,12:i:-). 

Purpose: To evaluate several key performance characters of this assay, including inclusivity, exclusivity, limited of detection (LOD), and food and environmental testing. 

Methods: Inclusivity was evaluated using 165 strains of Enteritidis and 335 strains of Typhimurium isolated from diverse geographic locations and sources, and 524 strains of non-target Salmonella (representing 166 serovars) and 30 non-Salmonella strains were used for exclusivity. For LOD, ground beef enrichments were artificially spiked with known concentrations of Salmonella and serially diluted for testing. For food and environmental testing, pre- or post-enrichment spiked samples of chicken breast, chicken rinse and chicken house swabs were used. 

Results: The assay demonstrated >99.4% inclusivity and 100% exclusivity. LOD was estimated at ≤104 CFU/ml after enrichment. The sensitivity of the assay was shown to be equivalent to a Real-Time PCR Assay for Salmonella in the food and environmental samples tested.

Significance: The results of these studies demonstrated that the novel system method is a fast, simple and accurate method for identifying Salmonella Enteritidis and Typhimurium in food and environmental samples. Furthermore, the assay shares identical PCR cycling parameters with other BAX® System real-time assays, allowing detection of multiple pathogens in a single PCR run in the BAX® System Q7 instrument.

Back to: Poster Session - Pathogens, Epidemiology, Novel Laboratory Methods, Food Defense, Communication Outreach and Education, General Microbiology, Dairy and Other Food Commodities, Food Toxicology
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