Purpose: This study was designed to determine concentrations of gluten in foods labeled as ‘gluten-free’ available in United States.
Methods: Seventy-eight samples labeled as ‘gluten-free’ were collected and analyzed using a gliadin competitive enzyme-linked immunosorbent assay (ELISA). The calculation of gluten is based on the assumption of the same ratio between gliadin and glutenin.
Results: Forty-eight of the 78 (61.5%) samples contained less than the limit of quantification (LOQ) of 10 mg/kg for gluten. Fourteen of the 78 (17.9%) labeled as ‘gluten-free’ samples contained less gluten than the guidelines established by the Codex guide line for gluten-free labeling (20 mg/kg). Sixteen samples (20.5%) did contain gluten levels ≥ 20 mg/kg, with gluten levels ranging from 20.3 mg/kg to 60.3 mg/kg; in particular, 5 out of 8 breakfast cereal samples showed gluten contents higher than 20 mg/kg.
Significance: These results are alarming as gluten sensitivity is known to vary among CD patients.