P2-32 Identifying the Accuracy of Non-O157:H7 Shiga Toxin-producing Escherichia coli Testing: A Proficiency Testing Study

Tuesday, August 5, 2014
Exhibit Hall D (Indiana Convention Center)
Christopher Snabes, American Proficiency Institute, Traverse City, MI
Daniel Edson, American Proficiency Institute, Traverse City, MI
Sue Empson, American Proficiency Institute, Traverse City, MI
Heather Jordan, American Proficiency Institute, Traverse City, MI
Susan Styles, American Proficiency Institute, Traverse City, MI
Introduction: While testing for Escherichia coli O157:H7 has been required for many years, in September 2011 the U.S. Department of Agriculture declared the following non-O157:H7 Shiga toxin-producing Escherichia coli (STEC) strains as adulterants: O26, O45, O103, O111, O121, and O145.  Mandatory testing of beef trim for the six additional STEC strains began in June 2012, and testing for these adulterants is novel to U.S. food laboratories.  With this mandatory testing comes the need for proficiency testing (PT).  The American Proficiency Institute (API), a food PT provider accredited by A2LA to ISO/IEC 17043, offers the only accredited PT program for all seven STEC adulterants.

Purpose: This PT study includes data from three test events in 2013, where 128 participants submitted results for detection and identification of an unknown STEC. Our objective was to assess whether laboratories can properly detect and identify STEC adulterants.

Methods: Responses for the seven STECs were recorded from 254 cumulative proficiency testing results submitted in three test events in the year 2013.  Participants were allowed to test the PT sample using the method of their choice and were asked to report the method used.

Results: Laboratories accurately detected an STEC present in a sample 96.1% of the time.  When the laboratory attempted to identify the strain, 95.6% of identifications were correct.  However, 39.9% of responses indicated participants were unable to, or chose not to, identify the strain in a particular sample.

Significance: As laboratories gain experience in routine testing for these STEC adulterants, proficiency testing will be useful in assessing their ability to detect and identify STECs.  The current results indicate that a significant portion of laboratories may test for the presence of STECs without identifying the strain.  This may change as industry practices and laboratory test methods for identifying these strains continue to evolve.