Purpose: The aim of this study was to investigate the antimicrobial pattern and analyze the genomic sequence of quinolone-resistance determinant region (grdr) of gyrase A in Arcobacter butzleri isolates.
Methods: Thirty-nine of A. butzleri were isolated from chicken in Korea. The antimicrobial susceptibility of 39 A. butzleri isolates and A. butzleri ATCC 49616 was performed with 22 antimicrobial agents following the guideline of NCCLS. Also the qrdr of the A. butzleri gyrA genes were amplified and sequenced.
Results: More than 95% of A. butzleri isolates were susceptible for aminoglycosides including amikacin, gentamicin, tobramycin, kanamycin, linsmycin, streptomycin, and neomycin. However, all isolates showed resistance to penicillin, sulfamethoxazole, ampicillin, cephalothin, and vancomycin. Seventeen of A. butzleri isolates were resistant to quinolones including enrofloxacin and ciprofloxacin. The quinolones-resistant 17 isolates had point mutation of qrdrwhich substitute Serine at 53 positions into Isoleucine.
Significance: The amikacin, gentamicin, tobramycin, linsmycin, streptomycin, and neomycin could be effective antibiotics to treat A. butzleri infection. The point mutation of qrdr of A. butzleri strains was highly associated with resistance to quinolones.