P3-67 Efficacy of Various Electrolyzed Oxidizing Waters to Control Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 from Cattle Hides

Wednesday, August 6, 2014
Exhibit Hall D (Indiana Convention Center)
Ravirajsinh Jadeja, University of Georgia, Griffin, GA
Yen-Con Hung, University of Georgia, Griffin, GA
Introduction: Beef products contaminated with Salmonella spp. and Shiga toxin-producing Escherichia coli (STEC) are major causes of foodborne illness in the United States.  Many previous studies reported cattle hide as major source of above mentioned pathogens.   Therefore it is important to reduce levels of aforementioned pathogens it is important to reduce the bacterial contamination of the hide.   

Purpose: This study was designed to determine suitability of various Electrolyzed Oxidizing water in carcass/hide wash cabinet to reduce aerobic plate counts (AC) and Enterobacteriaceae (EC) from uninoculated fresh cattle hides and Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 from inoculated hides.  

Methods: Fresh hides were cut in to 60 by 30 cm pieces and subjected to a total of eight different treatment solutions; near neutral pH EO water (NEW- pH 6.5 at room temperature, 150 mg/l available chlorine), alkaline pH EO water (AEO- pH 11.6 at room temperature), hot alkaline pH EO water at 43°C (HAEO- pH 11.60), alkaline pH EO water spray followed by 150 mg/l available chlorine containing near neutral pH EO water spray (A- NEW- both at room temperature), BlitzTM(PAA, pH 3.02 at room temperature), 5% lactic acid (LA, pH 2.04 at room temperature), deionized water (W) and no treatment (Control).  All treatments were applied by spraying each treatment solution for 30s at 20 PSI, using a specially constructed hide/carcass wash cabinet.  

Results: Five percent lactic acid spray treatment was found to be the most effective treatment and achieved 2.10 ± 0.56, 2.70 ± 0.30, 2.75 ± 0.42 and 2.90 ± 0.66 log CFU/cm2 of AC, EC, E. coli O157:H7 and S. Typhimurium DT 104 reductions, respectively (limit of detection 70 CFU/100cm2).  All EO water treatments were equally effective in reducing all target microorganisms, except E. coli O157:H7 (P ≤ 0.05).  HAEO and A-NEW treatments yielded significant reduction of E. coli O157:H7 compared to other EO water treatments.  

Significance: These results indicate that various EO water treatments could become viable option to control pathogens on hide during slaughter process.