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P1-06 Rapid Detection of Non-O157 STEC Eschericha coli Using a Flow Cytometry-based Pathogen Detection System

Monday, August 4, 2014
Exhibit Hall D (Indiana Convention Center)
Karen Beers, MCA Services, Rogers, AR
John Ferguson, MCA Services, Rogers, AR
Melinda Miller, Vivione Biosciences, Pine Bluff, AR
David Caldwell, Vivione Biosciences, Pine Bluff, AR
Shawn Ramsaroop, Vivione Biosciences, Pine Bluff, AR
Peter Rubinelli, University of Arkansas, Fayetteville, AR
Si Hong Park, University of Arkansas, Fayetteville, AR
Peggy Cook, MCA Services, Rogers, AR
Steven Ricke, University of Arkansas, Fayetteville, AR
Introduction: Beef producers need a reliable rapid technology for Escherichia coli STEC testing. Current rapid technologies require several hours of enrichment and additional time on the rapid technology instrument.  A flow cytometry-based (FCB) system improves the turn-around for results with a quicker enrichment requiring only 6.5 hours enrichment for a 25-gram sample and 8.5 hours enrichment for a 375-gram sample, with only an additional 30 minute time to result after enrichment.

Purpose: The purpose of this study was to validate the FCB system for non-O157 STEC testing compared to the FSIS/USDA reference method. 

Methods: A total of 10 samples per non-O157 STEC strain (O26, O45, O103, O121, O111 and O145) were inoculated (5 ground samples and 5 trim samples) using a batch method.   Three sample groups were tested, a 25-gram sample using the FBC system, a 375-gram sample using the FCB system, and a 325-gram sample using FSIS/USDA reference method.   All samples were enriched and tested according to the methods standard operating procedure.  Every FBC sample was additionally culturally confirmed using the FSIS/USDA reference method. 

Results: A total of 60 ground samples and 60 trim samples were inoculated and tested using the FBC technology.  For the ground samples, 59 were positive on the FCB system.  All 59 also culturally confirmed out, and the one negative sample was negative by cultural confirmation.  For the trim samples, 40 were positive on the FBC system.  All 40 also culturally confirmed out, and the 20 negative samples were also negative by cultural confirmation.   

Significance: The results of the study show both sample types (ground and trim) had 100% sensitivity and 100% specificity using the FBC technology.  Based on this study, the FBC system (Rapid-B Pathogen Detection System, Vivione Biosciences) offers beef producers a faster method for the rapid detection of non-O157 STEC testing.

Back to: Poster Session - Applied Laboratory Methods, Produce, Low-water Activity, Beverages and Water
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