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P1 Poster Session - Applied Laboratory Methods, Produce, Low-water Activity, Beverages and Water

Monday, August 4, 2014: 10:00 AM-6:00 PM
Exhibit Hall D (Indiana Convention Center)
Authors will stand at posters during coffee breaks, and are available then to discuss their research with conference attendees Poster in this session will be on display from 10:00 a.m. - 6:00 p.m. on Monday August 4. Presenting authors will stand by their posters to discuss their research according to this schedule: Posters Numbered P1-01 through P1-94: 10:00-11:30 a.m. and 5:00-6:00 p.m. Posters Numbered P1-95 through P1-189: 2:00-3:30 p.m. and 5:00-6:00 p.m.

Presentations

P1-01
Evaluation of Commercial Test Kits for Detection of Escherichia coli O157:H7 in Alfalfa Sprout Spent Irrigation Water
Nicole Maks, Illinois Institute of Technology; Christina Megalis, U.S. Food and Drug Administration; Tong-Jen Fu, U.S. Food and Drug Administration
P1-02
Evaluation of Novel Phage Protein Derived Latex Agglutination Assays for the Confirmation of the Shiga Toxin-producing Escherichia coli (STEC) Serogroups
Hari Prakash Dwivedi, bioMérieux; Beth Osia, bioMérieux; Gregory Devulder, bioMérieux; Jean-Louis Pittet, bioMérieux
P1-03
Use of Specific CRISPR-2 Spacers for the Detection of the Big Six STEC Serotypes by Real-time PCR
Solomon Gebru, U.S. Food and Drug Administration; Mark Mammel, U.S. Food and Drug Administration; Christopher Elkins, U.S. Food and Drug Administration
P1-04
Evaluation of NanoLuc® and GFP Reporter-labeled Control Strains for Shiga Toxin-producing Escherichia coli (STEC) Assays
Jodie Lee, ATCC; Dev Mittar, ATCC
P1-05
Comparison of chromID EHEC, Modified Rainbow Agar, and Rainbow Agar for the Isolation and Confirmation of USDA Top 7 Shigatoxigenic Escherichia coli from Pure Culture and Beef Trim Enrichment Using FSIS MLG 5.06 and MLG 5B.03 Methods
Leslie Thompson, AEGIS Food Testing Laboratories; Cole Liska, AEGIS Food Testing Laboratories; Carol Valenzuela Martinez, AEGIS Food Testing Laboratories
P1-06
Rapid Detection of Non-O157 STEC Eschericha coli Using a Flow Cytometry-based Pathogen Detection System
Karen Beers, MCA Services; John Ferguson, MCA Services; Melinda Miller, Vivione Biosciences; David Caldwell, Vivione Biosciences; Shawn Ramsaroop, Vivione Biosciences; Peter Rubinelli, University of Arkansas; Si Hong Park, University of Arkansas; Peggy Cook, MCA Services; Steven Ricke, University of Arkansas
P1-07
Evaluation of Commercial Agar for the Detection of Shiga Toxin-producing Escherichia coli
Gentry Lewis, University of Nebraska-Lincoln; Zachary Stromberg, University of Nebraska-Lincoln; Rodney Moxley, University of Nebraska-Lincoln
P1-08
Evaluation of Enrichment and Transport Media for Detection and Enumeration of Shiga Toxin-producing Escherichia coli
Nicholas Baumann, Kansas State University; Aja West, Tuskegee University; Nicholas Sevart, Kansas State University; Donka Milke, Kansas State University; Randall Phebus, Kansas State University
P1-09
Detection and Isolation of Shiga Toxin-producing Escherichia coli (STEC) O104 and Other STEC Serogroups of Public Health Concern
Gian Marco Baranzoni, University of Bologna; Pina Fratamico, U.S. Department of Agriculture-ARS-ERRC; Fernando Rubio, Abraxis LLC; Thomas Glaze, Abraxis LLC; Natalie Launchi, U.S. Department of Agriculture-ARS-ERRC; Lori Bagi, U.S. Department of Agriculture-ARS-ERRC; Sabrina Albonetti, University of Bologna
P1-10
Antibiotic Resistance of Escherichia coli Strains Isolated from Avian Samples in the Region of Algiers (Algeria)
Taha Mossadak Hamdi, High Veterinary School of Algiers
P1-11
Differentiation of Colony Morphology of Shiga Toxin-producing Escherichia coli on Commercial Agar Media
Jisu Kang, University of Missouri-Columbia; Ami Yoo, University of Missouri-Columbia; Prashant Singh, University of Missouri-Columbia; Azlin Mustapha, University of Missouri-Columbia
P1-12
Multiplex Real-time PCR Assay for Detection of Eight STEC Serotypes
Prashant Singh, University of Missouri-Columbia; Azlin Mustapha, University of Missouri-Columbia
P1-13
MALDI-TOF MS-based Approach for Discrimination of Enterohemorrhagic Escherichia coli O157, O26 and O111
Teruyo Ojima-Kato, Aichi Science and Technology Foundation; Naomi Yamamoto, Meijo University; Hiroto Tamura, Meijo University
P1-14
Towards Development of Effective Interventions to Eliminate Escherichia coli during Carcass Chilling
Chawalit Kocharunchitt, University of Tasmania; Bianca Porteus, University of Tasmania; John Bowman, University of Tasmania; Ian Jenson, Meat & Livestock Australia; Tom Ross, University of Tasmania
P1-15
Rapid Typing of BIG 7 Shiga Toxin-producing Escherichia coli in Food by Genome Sequence Scanning
Srinivas Ramaswamy, Pathogenetix; Mohan Manoj Kumar, Pathogenetix; Ekaterina Protozanova, Pathogenetix; Shavana Ohneswere, Pathogenetix; Katarzyna Crissy, Pathogenetix; Meredith Waldron, Pathogenetix; Robert Meltzer, Pathogenetix; David Tims, Pathogenetix; Rudolf Gilmanshin, Pathogenetix
P1-16
Detecting Escherichia coli O157:H7 in Composite 375 g Raw Ground Beef Samples Using the DuPont™ BAX® System Method
Steven Hoelzer, DuPont Nutrition & Health; Julie Weller, DuPont Nutrition & Health; Nisha Corrigan, DuPont Nutrition & Health; Stacy Stoltenberg, DuPont Nutrition & Health; Morgan Wallace, DuPont Nutrition & Health; Jacqueline Harris, DuPont Nutrition & Health; Krystal Shortlidge, DuPont Nutrition & Health
P1-17
Detection of Escherichia coli O157H:7 and Non-O157 Shiga Toxin-producing E. coli by Triplex Real-time PCR
Weimin Wang, U.S. Food and Drug Administration; Huanli Liu, U.S. Food and Drug Administration; Christopher Elkins, U.S. Food and Drug Administration; Baoguang Li, U.S. Food and Drug Administration
P1-18
Development of an Internal Standard Approach for Comparing Flow Cytometry-based (FCB) Pathogen Detection System (Rapid-B Pathogen Detection System, Vivione Biosciences) and Quantitative PCR for Enumeration of Foodborne Pathogenic Escherichia coli
Peter Rubinelli, University of Arkansas; Si Hong Park, University of Arkansas; Melinda Miller, Vivione Biosciences; David Caldwell, Vivione Biosciences; Shawn Ramsaroop, Vivione Biosciences; Karen Beers, MCA Services; Peggy Cook, MCA Services; Steven Ricke, University of Arkansas
P1-19
The Assessment of Escherichia coli as an Indicator of Microbial Quality of Irrigation Waters Used for Produce
Natalie Brassill, University of Arizona; Kelly Bright, The University of Arizona; Akrum Tamimi, University of Arizona; Kurt Nolte, University of Arizona; Charles Gerba, University of Arizona; Channah Rock, University of Arizona
P1-20
Evaluation of ChromID® EHEC Agar for Detection of Seven Major Serogroups of Shiga Toxin-producing Escherichia coli from Cattle Feces
Lance Noll, Kansas State University; Pragathi Shridhar, Kansas State University; William Baumgartner, Kansas State University; Xiaorong Shi, Kansas State University; TG Nagaraja, Kansas State University
P1-21
Evaluation of Different Immuno-magnetic Beads and Selective Plating Media for the Confirmation of Shiga Toxin-producing Escherichia coli Detected by Molecular Methods in Beef Trim Enrichment Broths
Joseph Bosilevac, U.S. Department of Agriculture-ARS; Brandon Luedtke, U.S. Department of Agriculture-ARS-USMARC; Pablo Rovira, Instituto Nacional de Investigacion Agropecuaria (INIA)
P1-22
Validation of a New and Innovative Rapid Assay for the Detection of Salmonella in Food and Environmental Samples
Melissa Buzinhani, FoodChek Laboratories Inc.; Renaud Tremblay, FoodChek Laboratories Inc.; Michael Giuffre, FoodChek Systems Inc.; Gabriela Martinez, FoodChek Laboratories Inc.
P1-23
Evaluation of 3M™ Molecular Detection System (MDS) for the Rapid Detection of Salmonella spp. on Duck Wings, Bean Sprouts and Fish Balls
Hazel Sin Yue Lim, National University of Singapore; Marta Mikš-Krajnik, National University of Singapore; Qianwang Zheng, National University of Singapore; Matthew Turner, 3M Asia Pacific; Hyun-Gyun Yuk, National University of Singapore
P1-24
Performance of Techniques for Identification of Serotypes of Salmonella
Audecir Giombelli, Universidade Federal de Minas Gerais; Elton Rodrigo Cê, Universidade Tecnológica Federal do Paraná; M. Beatriz Gloria, Universidade Federal De Minas Gerais
P1-25
Accuracy of Molecular Screening Methods for the Detection of Salmonella enterica in Production Ground Poultry Samples
W. Evan Chaney, Roka Bioscience, Inc.; Tyler Stephens, Roka Bioscience, Inc.; Joseph Kibala, Roka Bioscience, Inc.; Tanushree Shah, Roka Bioscience, Inc.; Erin Dreyling, Roka Bioscience, Inc.
P1-26
Accuracy of Molecular Screening Methods for the Detection of Salmonella enterica in Production Poultry Rinse Samples
W. Evan Chaney, Roka Bioscience, Inc.; Tyler Stephens, Roka Bioscience, Inc.; Joseph Kibala, Roka Bioscience, Inc.; Tanushree Shah, Roka Bioscience, Inc.; Erin Dreyling, Roka Bioscience, Inc.
P1-27
Method Verification for the Detection of Salmonella enterica by the Atlas® Salmonella G2 (SG2) Detection Assay in Produce Matrices
Tyler Stephens, Roka Bioscience, Inc.; W. Evan Chaney, Roka Bioscience, Inc.; Joseph Kibala, Roka Bioscience, Inc.; Tanushree Shah, Roka Bioscience, Inc.; Erin Dreyling, Roka Bioscience, Inc.
P1-28
Evaluation of the 3M™ Petrifilm Salmonella Express System for the Detection of Salmonella in Thailand Beverages
Saengrawee Jongvanich, 3M Food Safety, 3M Thailand Ltd.; Wannee Margun, Ministry of Public Health; Janejira Fuangpaiboon, 3M Food Safety, 3M Thailand Ltd.; Patcharaporn Kaewsumran, Ministry of Public Health; Nongnuch Promla, 3M Food Safety, 3M Thailand Ltd.
P1-29
Evaluation of the 3M™ Molecular Detection Assay (MDA) Salmonella for the Detection of Salmonella in 125 g of Thai Vegetables
Patchana Supasoon, Department of Agriculture; Suphakhinee Jeananankul, Department of Agriculture; Thamolwan Laovittayanurak, 3M Food Safety, 3M Thailand Ltd.; Nongnuch Promla, 3M Food Safety, 3M Thailand Ltd.
P1-30
Comparison between 3M™ Petrifilm™ Salmonella Express System and ISO 6579 Conventional Method for the Detection of Salmonella spp. in Cook-chill and Cook-freeze Retail Foods in Bangkok, Thailand
Aphacha Jindaprasert, King Mongkut’s Institute of Technology; Asanee Vichitraka, King Mongkut’s Institute of Technology; Janejira Fuangpaiboon, 3M Food Safety, 3M Thailand Ltd.; Nongnuch Promla, 3M Food Safety, 3M Thailand Ltd.; Adisorn Swetwiwathana, King Mongkut’s Institute of Technology
P1-31
Performance of a New Molecular Platform for the Detection of Listeria monocytogenes in Thai Seafood Matrices
Wikrommanas Auawithoothij, Samutsakorn Fish Inspection and Research Center; Thamolwan Laovittayanurak, 3M Food Safety, 3M Thailand Ltd.; Nongnuch Promla, 3M Food Safety, 3M Thailand Ltd.; Wanida Mukkana, 3M Food Safety, 3M Thailand Ltd.; Panida Pisaisawat, 3M Food Safety, 3M Thailand Ltd.
P1-32
Evaluation of a New Low-cost Multicolor Fluorescence Capillary Electrophoresis System for Multiple-locus Variable-number Tandem-repeat Analysis (MLVA) of Salmonella
Hyun Joong Kim, Iowa State University; Byron Brehm-Stecher, Iowa State University
P1-33
The ANSR® Salmonella Assay for the Detection of Salmonella spp. in Pasteurized Egg Products
Lei Zhang, Neogen Corporation; Debi Foti, Neogen Corporation; Preetha Biswas, Neogen Corporation; Mark Mozola, Neogen Corporation; Jennifer Rice, Neogen Corporation
P1-34
Heat Resistance Stability of Enterococcus faecium NRRL B-2354 and Salmonella Enteritidis PT30 on Almonds during Extended Storage at 4 and 25°C
Shirin Abd, The National Food Lab; Carrie Ferstl, The National Food Laboratory, Inc.
P1-35
Pulsed-field Gel Electrophoresis Subtyping for Salmonella Serotype Discrimination
Diana Ayala, Texas Tech University; Kendra Nightingale, Texas Tech University; Mindy Brashears, Texas Tech University
P1-36
A Comprehensive Comparison of the BAX® System Salmonella 2 and Real-time Salmonella PCR Assays to the FDA BAM Reference Methods for the Detection of Salmonella in a Variety of Soy Ingredients
Tamrat Belete, Solae; Erin Crowley, Q Laboratories, Inc.; Patrick Bird, Q Laboratories, Inc.; Joseph Gensic, DuPont Nutrition and Health; Morgan Wallace, DuPont Nutrition & Health
P1-37
Analysis of Salmonella spp. Enrichment for Foodborne Pathogen Detection
Kirsten Hirneisen, U.S. Food and Drug Administration; Chorng-Ming Cheng, U.S. Food and Drug Administration; Donna Williams-Hill, U.S. Food and Drug Administration
P1-38
A Novel Real-time PCR Method for Rapid Molecular Detection and Serotyping of Salmonella Typhimurium
Hélène Frenkiel-Lebossé, Bio-Rad; Fanny Margotteau, Bio-Rad Laboratories; Lydie Réhault, Bio-Rad Laboratories; Jean-Philippe Tourniaire, Bio-Rad Laboratories; Jean-Pierre Facon, Bio-Rad Laboratories; Sophie Pierre, Bio-Rad Laboratories; Jean-Francois Mouscadet, Bio-Rad Laboratories
P1-39
Multiplex PCR for Simultaneous Identification of 8 Common Salmonella Serovars
Shuang Wu, University of Florida; Charles Clines, Arkansas State University; Stephanie Myal, Arkansas State University; Soohyoun Ahn, University of Florida
P1-40
Detection of Salmonella species by the DuPont™ BAX® System Real-time PCR Assay for Salmonella: Collaborative Study
Morgan Wallace, DuPont Nutrition & Health; Bridget Andaloro, DuPont Nutrition & Health; Dawn Fallon, DuPont Nutrition & Health; Nisha Corrigan, DuPont Nutrition & Health; Stephen Varkey, DuPont Nutrition & Health; Daniel DeMarco, DuPont Nutrition & Health; Steven Hoelzer, DuPont Nutrition & Health; Julie Weller, DuPont Nutrition & Health; George Tice, DuPont Nutrition & Health; Patrick Bird, Q Laboratories, Inc.; Erin Crowley, Q Laboratories, Inc.; Jonathan Flannery, Q Laboratories, Inc.; Kiel Fisher, Q Laboratories, Inc.; Travis Huffman, Q Laboratories, Inc.; Megan Boyle, Q Laboratories, Inc.; M. Joseph Benzinger, Q Laboratories, Inc.; Paige Bedinghaus, Q Laboratories, Inc.; Katherine Goetz, Q Laboratories, Inc.; William Judd, Q Laboratories, Inc.; James Agin, Q Laboratories, Inc.; David Goins, Q Laboratories, Inc.; Andrew Farnum, DuPont Nutrition & Health; Jeffrey Rohrbeck, DuPont Nutrition & Health; Eugene Davis, DuPont Nutrition & Health
P1-41
Rapid Serotyping Method to Characterize Salmonella enterica subsp. enterica Isolates
Christina Ferrato, Provincial Laboratory for Public Health - Alberta; Linda Chui, Provincial Laboratory for Public Health - Alberta; Marie Louie, Provincial Laboratory for Public Health - Alberta
P1-43
Pathatrix Auto™ - the First AFNOR-approved Real-time PCR Method for Detecting Salmonella in Pooled Food Samples
Jason Wall, Life Technologies, Inc.; Daniele Sohier, ADRIA Développement; Rick Conrad, Life Technologies, Inc.
P1-44
Detection of Newly Described Listeria Species by Three Chromogenic Agars and One Real-time PCR Method
Christophe Quiring, Bio-Rad Laboratories; Rebecca Dièvart, Bio-Rad Laboratories; Jean-Francois Mouscadet, Bio-Rad Laboratories
P1-45
Development and Preliminary Evaluation of a Real-time PCR Assay for the Detection of Listeria monocytogenes
Seth Blumerman, DuPont Nutrition & Health; Lois Fleck, DuPont Nutrition & Health; Daniel DeMarco, DuPont Nutrition & Health; Stephen Varkey, DuPont Nutrition & Health; Mark Jensen, DuPont Nutrition & Health; Andrew Farnum, DuPont Nutrition & Health; Teresa Brodeur, DuPont Nutrition & Health; Timothy Dambaugh, DuPont Nutrition & Health; Gongbo Wang, DuPont Nutrition & Health; George Tice, DuPont Nutrition & Health
P1-46
An Alternative Tool for Monitoring the Presence of Listeria spp. in the Industrial Environment
Sergiy Olishevskyy, FoodChek Laboratories Inc.; Sylvain Fournaise, Olymel SEC/LP; Michael Giuffre, FoodChek Systems Inc.; Gabriela Martinez, FoodChek Laboratories Inc.
P1-47
Comparison of Two Selective Media and Validation of Conventional and Real-time PCR Assays for the Detection of Listeria monocytogenes in Food Matrices
Dong-Hyeon Kim, Konkuk University; Jung-Whan Chon, Konkuk University; Dasom Choi, Konkuk University; Young-Ji Kim, Konkuk University; Hong-Seok Kim, Konkuk University; Jin San Moon, Animal, Plant and Fisheries Quarantine and Inspection Agency; Kun-Ho Seo, Konkuk University
P1-48
Modification of Bolton Broth by Supplementation of Triclosan for the Isolation of Campylobacter jejuni and Campylobacter coli in Chicken Carcass Rinse
Jung-Whan Chon, Konkuk University; Hong-Seok Kim, Konkuk University; Dong-Hyeon Kim, Konkuk University; Kun-Ho Seo, Konkuk University
P1-49
An Independent Laboratory Evaluation of MericonTM Listeria and L. monocytogenes Detection Kits
Kiel Fisher, Q Laboratories, Inc.; Patrick Bird, Q Laboratories, Inc.; Erin Crowley, Q Laboratories, Inc.; Jonathan Flannery, Q Laboratories, Inc.; Marcia Armstrong, QIAGEN GmbH; Kathrin Wolf, QIAGEN GmbH; Sarah Fakih, QIAGEN GmbH; Ralf Peist, QIAGEN GmbH
P1-50
Use of the RapidChek® Listeria F.A.S.T. Test System to Detect Low Levels of Listeria Species in Composite Environmental Sponge Samples
Zheng Jiang, Romer Labs
P1-51
Comparative Validation Study to Demonstrate the Equivalence of an 8-hour Dual Salmonella and E. coli O157:H7 Enrichment for Assurance GDS® to Culture Methods for the Detection of Salmonella in Selected Foods and Environ
David Kerr, BioControl Systems, Inc.; Geoff Bright, BioControl Systems, Inc.
P1-52
Comparison of Testing Efficiency among Three Rapid Pathogen Detection Platforms
Christian Blyth, 3M Canada Corporation
P1-53
Use of Non-proprietary Harmonized Enrichment Media for the Detection of Escherichia coli O157:H7, Non-O157 STEC, and Salmonella in 375 g Beef and Produce Samples with the Atlas® System
Anja Bubeck-Barrett, Roka Bioscience, Inc.; Bettina Groschel, Roka Bioscience, Inc.; Kristin Livezey, Roka Bioscience, Inc.; Hua Yang, Roka Bioscience, Inc.; William Kwong, Roka Bioscience, Inc.; Apolonia Huerta, Roka Bioscience, Inc.; Celina Puente, Roka Bioscience, Inc.; Steve Vaughn, Roka Bioscience, Inc.; Brett Maroni, Roka Bioscience, Inc.; Kevin Tsao, Roka Bioscience, Inc.; Julian Kougl, Roka Bioscience, Inc.; Michele Wisniewski, Roka Bioscience, Inc.; Joe Garcia, Roka Bioscience, Inc.; Bernadine Luong, Roka Bioscience, Inc.; Edgar Kamantique, Roka Bioscience, Inc.; Chad Fleischer, Roka Bioscience, Inc.; Brett Weaver, Roka Bioscience, Inc.; Jarrod Morgan, Roka Bioscience, Inc.; Michael Reshatoff, Roka Bioscience, Inc.; Michael Becker, Roka Bioscience, Inc.; Erin Crowley, Q Laboratories, Inc.; Patrick Bird, Q Laboratories, Inc.; Benjamin Bastin, Q Laboratories, Inc.
P1-54
Real-time Multiplex Detection of Escherichia coli O157:H7 and Salmonella in Raw Non-intact Beef Using Housekeeping Genes as Targets
Samantha Stewart, Texas Tech University
P1-55
Development and Application of Selective Enrichment Broth (CES) for Simultaneous Growth of Cronobacter sakazakii, Escherichia coli O157:H7 and Salmonella spp
Se-Ri Kim, Rural Development Administration; Min-Kyoung Seo, Rural Development Administration; Won-Il Kim, Rural Development Administration; Kyoung-Yul Ryu, Rural Development Administration; Byung Seok Kim, Rural Development Administration; Hwang-Yong Kim, Rural Development Administration; Jae-Gee Ryu, Rural Development Administration
P1-56
A Robust Multiplex Real-time PCR Method for Simultaneous Detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes in Fresh Fruits and Vegetables
Venugopal Sathyamoorthy, U.S. Food and Drug Administration; Larisa Trach, U.S. Food and Drug Administration; Yiping He, U.S. Department of Agriculture-ARS-ERRC; Ben Tall, U.S. Food and Drug Administration; Hannah Chase, U.S. Food and Drug Administration; Seongeun Hwang, U.S. Food and Drug Administration; Boram Lee, U.S. Food and Drug Administration; Barbara McCardell, U.S. Food and Drug Administration; Atin Datta, U.S. Food and Drug Administration
P1-57
Chitosan-based Antibacterial Microparticles: Mode of Action in the Control of Pathogens Associated with Fresh Produce
Tsz-wai Tsui, Rutgers University; Karl Matthews, Rutgers University; Nina Shapley, Rutgers University; Anubhav Tripathi, Brown University
P1-58
Sanitizer Efficacy Protocol Using a Dried Microbial Model
Charles Giambrone, Rochester Midland Corporation; Griffin Jadwin, Rochester Midland Corporation; Richard Moll, Rochester Midland Corporation
P1-59
Development and Application of Novel SNP-based Serotyping Assays in Targeting Salmonella enterica within the Poultry Production and Processing Continuum
Michael Rothrock, U.S. Department of Agriculture-ARS; Jean Guard, U.S. Department of Agriculture; Richard Gast, U.S. Department of Agriculture-ARS-ESQRU
P1-60
Viability PCR: An Evaluation of Propidium Monoazide for Live/Dead Differentiation of Microorganisms
Kathrin Wolf, QIAGEN GmbH; Sarah Fakih, QIAGEN GmbH; Corinna Küppers, QIAGEN GmbH; Annette Bogdoll, QIAGEN GmbH; Sabine Schlappa, QIAGEN GmbH; Marcia Armstrong, QIAGEN GmbH; Sandra Luley, QIAGEN GmbH; Ralf Peist, QIAGEN GmbH
P1-61
Single Laboratory Validation of a Vibrio TaqMan qPCR Assay for Identification of Vibrio Isolates
Paul Browning, New Mexico State University; Lyssa White, New Mexico State University; Juan Olea, New Mexico State University; Ruiqing Pamboukian, U.S. Food and Drug Administration; Ruben Zapata, New Mexico State University; Willis Fedio, New Mexico State University
P1-62
Comparison of Dehydrated Film Media for the Quantitative Enumeration of Yeasts and Molds
Sailaja Chandrapati, 3M Food Safety; Tera Nordby, 3M Food Safety; Micki Rosauer, 3M Food Safety
P1-63
Rapid Resin-based Method for Concentration of Rotavirus, Hepatitis A Virus and Adenovirus 40 from Tap Water
Alma Perez-Mendez, Colorado State University; Jeffrey Chandler, University of Wyoming; Bledar Bisha, University of Wyoming; Shannon Coleman, Colorado State University; Lawrence Goodridge, Colorado State University
P1-64
Detection of Viable Murine Norovirus Using Plaque Assay and Propidium Monoazide Real-time Reverse Transcription-polymerase Chain Reaction
Min Hwa Lee, Chung-Ang University; Dong Joo Seo, Chung-Ang University; Jina Seo, Chung-Ang University; Su Been Jeon, Chung-Ang University; Hyejin Oh, Chung-Ang University; Changsun Choi, Chung-Ang University
P1-65
Enhanced Reverse Transcription Polymerase Chain Reaction Enzyme-linked Immunosorbent Assay (RT-PCR-ELISA) for Simultaneous Detection of Genogroup I and Genogroup II Noroviruses
Su Been Jeon, Chung-Ang University; Dong Joo Seo, Chung-Ang University; Min Hwa Lee, Chung-Ang University; Jina Seo, Chung-Ang University; Hyejin Oh, Chung-Ang University; Changsun Choi, Chung-Ang University
P1-66
Use of Kidney Inhibition Swab Test to Evaluate Antimicrobial Residues in Pork Kidney from a Market Survey in Fargo, North Dakota
Weilin Shelver, U.S. Department of Agriculture-ARS; Amy McGarvey, U.S. Department of Agriculture-ARS; Erin Loeb, Custer Health
P1-67
Meat Species Identification Using PCR-RFLP and Native Capillary Electrophoresis System
Mirjana Kozulic, QIAGEN Instruments AG; Renaud Cassier, ADGENE Laboratoire; Marcia Armstrong, QIAGEN GmbH
P1-68
Evolution of Microbiological Analytical Methods for Dairy Industry Needs
Daniele Sohier, ADRIA Développement; Sonia Pavan, ADRIA Développement; Armelle Riou, ADRIA Développement; Jerome Combrisson, Danone Research; Florence Postollec, ADRIA UMT14.01 SPORE RISK
P1-69
Leuconostoc mesenteroides subsp. mesenteroides: Bacteriocinogenic Strain Isolated from Brazilian Water-buffalo Mozzarella Cheese
Aline Paula, UNESP; Ana Beatriz Jeronymo-Ceneviva, UNESP; Svetoslav Todorov, Universidade de Sao Pãulo; Yvan Choiset, INRA; Jean-Marc Chobert, INRA; Thomas Haertlé, INRA; Xavier Dousset, ONIRIS; Ana Lucia Barretto Penna, Universidade Estadual Paulista
P1-70
Evaluation of the 3M™ Microbial Luminescence System (MLS II) for Microbial Detection in Flavored UHT Milk
Alejandra Diaz, Hersheys México; April Pontius-Peters, Hersheys; Pedro Duran, 3M Food Safety; Daniela Fernandez, 3M Food Safety; Guadalupe Mondragon, 3M Food Safety; Gustavo Gonzalez-Gonzalez, 3M Food Safety
P1-71
Development and Inter-laboratory Validation of Aflatoxin M1 Analysis in Bovine Milk
Yang Chen, U.S. Food and Drug Administration; Ramesh Yettella, Institute for Food Safety and Health; Salvador Lopez, Institute for Food Safety and Health; Ravinder Reddy, U.S. Food and Drug Administration
P1-72
A New Rapid Quantitative Method for Detection of Aerobic Plate Count from Environmental Hygiene Swabs
Joellen Feirtag, University of Minnesota; Daniel Smith, Mocon Inc.; Alan Traylor, MOCON, Inc.
P1-73
ISO 11133 – Evaluation of Microbiological Performance Testing in Quality Control of Culture Media
Barbara Gerten, Merck Millipore; Vera Krüger, Merck KGaA; Rainer Schrod, Merck KGaA; Stefanie Fischer, Merck KGaA
P1-74
Development and Validation of a Rapid Test Kit for Detection of Pork Meat Residues
Brianda Barrios-Lopez, IEH Laboratories & Consulting Group; Nick Becker, IEH Laboratories & Consulting Group; Jongkit Masiri, IEH Laboratories & Consulting Group; Jeffrey Day, IEH Laboratories & Consulting Group; Alex Agapov, IEH Laboratories & Consulting Group; Lora Benoit, IEH Laboratories & Consulting Group; Mahzad Meshgi, IEH Laboratories & Consulting Group; Cesar Nadala, IEH Laboratories & Consulting Group; Mansour Samadpour, IEH Laboratories & Consulting Group
P1-75
Development and Validation of a Rapid Qualitative Test Kit for Detection of Horse Meat Residues
Brianda Barrios-Lopez, IEH Laboratories & Consulting Group; Nick Becker, IEH Laboratories & Consulting Group; Jongkit Masiri, IEH Laboratories & Consulting Group; Jeffrey Day, IEH Laboratories & Consulting Group; Alex Agapov, IEH Laboratories & Consulting Group; Lora Benoit, IEH Laboratories & Consulting Group; Mahzad Meshgi, IEH Laboratories & Consulting Group; Cesar Nadala, IEH Laboratories & Consulting Group; Mansour Samadpour, IEH Laboratories & Consulting Group
P1-76
Development and Validation of a Novel Gluten Detection Lateral-flow Device
Brianda Barrios-Lopez, IEH Laboratories & Consulting Group; Jongkit Masiri, IEH Laboratories & Consulting Group; Nick Becker, IEH Laboratories & Consulting Group; Mahzad Meshgi, IEH Laboratories & Consulting Group; Lora Benoit, IEH Laboratories & Consulting Group; Cesar Nadala, IEH Laboratories & Consulting Group; Mansour Samadpour, IEH Laboratories & Consulting Group
P1-77
Development and Characterization of Novel Monoclonal Antibodies Directed against Cereal Prolamins
Brianda Barrios-Lopez, IEH Laboratories & Consulting Group; Jennifer Yamaura, IEH Laboratories & Consulting Group; Xiaoqiong Huang, IEH Laboratories & Consulting Group; Lora Benoit, IEH Laboratories & Consulting Group; Jeffrey Day, IEH Laboratories & Consulting Group; Cesar Nadala, IEH Laboratories & Consulting Group; Mansour Samadpour, IEH Laboratories & Consulting Group
P1-78
A Novel Universal Extraction Buffer for Use in Immunoassay-based Detection of Food Allergens and Gluten
Brianda Barrios-Lopez, IEH Laboratories & Consulting Group; Mahzad Meshgi, IEH Laboratories & Consulting Group; Jongkit Masiri, IEH Laboratories & Consulting Group; Lora Benoit, IEH Laboratories & Consulting Group; Cesar Nadala, IEH Laboratories & Consulting Group; Mansour Samadpour, IEH Laboratories & Consulting Group
P1-79
Development and Validation of a Gluten ELISA Kit Using Polyclonal Antibodies Configured in Sandwich Format
Brianda Barrios-Lopez, IEH Laboratories & Consulting Group; Mahzad Meshgi, IEH Laboratories & Consulting Group; Jongkit Masiri, IEH Laboratories & Consulting Group; Lora Benoit, IEH Laboratories & Consulting Group; Nick Becker, IEH Laboratories & Consulting Group; Jeffrey Day, IEH Laboratories & Consulting Group; Cesar Nadala, IEH Laboratories & Consulting Group; Mansour Samadpour, IEH Laboratories & Consulting Group
P1-80
The Development and Validation of a Novel Gluten ELISA Kit Based on Monoclonal Antibodies Configured in Competitive Format
Brianda Barrios-Lopez, IEH Laboratories & Consulting Group; Mahzad Meshgi, IEH Laboratories & Consulting Group; Jongkit Masiri, IEH Laboratories & Consulting Group; Lora Benoit, IEH Laboratories & Consulting Group; Nick Becker, IEH Laboratories & Consulting Group; Jeffrey Day, IEH Laboratories & Consulting Group; Cesar Nadala, IEH Laboratories & Consulting Group; Mansour Samadpour, IEH Laboratories & Consulting Group
P1-81
A Novel Extraction Buffer for Use in Gluten Immunoassays
Brianda Barrios-Lopez, IEH Laboratories & Consulting Group; Jongkit Masiri, IEH Laboratories & Consulting Group; Mahzad Meshgi, IEH Laboratories & Consulting Group; Lora Benoit, IEH Laboratories & Consulting Group; Nick Becker, IEH Laboratories & Consulting Group; Cesar Nadala, IEH Laboratories & Consulting Group; Mansour Samadpour, IEH Laboratories & Consulting Group
P1-82
Validation of the First Marketed Multiplex Real-time PCR Rapid Test for Salmonella Enteritidis and Typhimurium Detection
Christina Harzman, BIOTECON Diagnostics; Hanna Hartenstein, BIOTECON Diagnostics; Cordt Groenewald, BIOTECON Diagnostics; Kornelia Berghof-Jaeger, BIOTECON Diagnostics
P1-83
Identification of Leafy Green Recovered Bacteria with Antagonistic Activity against Enteric Bacterial Pathogens
Keila Perez, Texas A&M University; Mariana Villarreal-Silva, Texas A&M University; Mustafa Akbulut, Texas A&M University; Luis Cisneros-Zevallos, Texas A&M University; Matthew Taylor, Texas A&M University; Alejandro Castillo, Texas A&M University
P1-84
Microbial Assessment of Leafy Greens and Leafy Greens Contact Surfaces in Retail Foodservice Operations
Angela Shaw, Iowa State University; Lakshman Rajagopal, Iowa State University; Catherine Strohbehn, Iowa State University; Kevin Sauer, Kansas State University; Susan Arendt, Iowa State University
P1-85
Effects of Plant Growth Promoting Rhizobacteria on the Immune Response of Romaine Lettuce and Spinach toward Human Bacterial Pathoges
Sarah Markland, University of Delaware; Harsh Bais, University of Delaware; Kalmia Kniel, University of Delaware
P1-86
Persistence of Eschericha coli on Romaine Lettuce in Protected Agricultural Environments
Kenneth Shenge, The Ohio State University; Jeffrey LeJeune, The Ohio State University
P1-87
Fate of Salmonella enterica in a Mixed Ingredient Salad Containing Lettuce, Cooked Chicken and Cheese
Federica Bovo, University of Bologna; Alessandra de Cesare, University of Bologna; Gerardo Manfreda, University of Bologna; Susan Bach, Agriculture and Agri-Food Canada; Pascal Delaquis, Agriculture and Agri-Food Canada
P1-88
­­­Survival of Norovirus Surrogates on Lettuce: A Comparison among Feline Calicivirus, Murine Norovirus, Porcine Sapovirus and Tulane Virus
Malak Esseili, The Ohio State University; Linda Saif, The Ohio State University; Tibor Farkas, Cincinnati Children’s Hospital Medical Center; Qiuhong Wang, The Ohio State University
P1-89
Effect of Different Mulches Used for Growing Organic Lettuce on Soil and Epiphytically-associated Fecal Indicator Bacteria
Aixia Xu, University of Maryland; Shirley Micallef, University of Maryland
P1-90
The Impact of Solids on the Efficacy of Chlorine in Preventing Salmonella Cross-contamination during Washing of Fresh-cut Lettuce
Xinhe Wang, Illinois Institute of Technology; Yusu Huang, Illinois Institute of Technology; David Laird, U.S. Food and Drug Administration-USPHS; Tong-Jen Fu, U.S. Food and Drug Administration
P1-91
Effectiveness of a Batch Ozonated Retail Wash System for Iceberg Lettuce
Chelsea Kaminski, University of California-Davis; Adrian Sbodio, University of CA; Jeremy Roland, University of California-Davis; Lindsay Derby, University of California-Davis; Trevor Suslow, University of CA
P1-93
Effectiveness of Listex™ (P100) against Listeria monocytogenes on Spinach Leaves
James Schnepf, California Polytechnic State University; Amanda Lathrop, California Polytechnic State University; Keith Vorst, California Polytechnic State University; Wyatt Brown, California Polytechnic State University
P1-94
Surface Texture Analysis of Fresh Produce using Optical Interferometry
James Schnepf, California Polytechnic State University; Amanda Lathrop, California Polytechnic State University; Derek Manheim, California Polytechnic State University
P1-95
Sensitivity of Pseudomonas fluorescens to Gamma Irradiation Following Surface Inoculations on Romaine Lettuce and Baby Spinach
Modesto Olanya, Agricultural Research Service; Brendan Niemira, U.S. Department of Agriculture-ARS; John Phillips, U.S. Department of Agriculture-ARS-NAA
P1-96
Seed Disinfection in Sprout Production: Survey of Methods Applied, Basis for Selection and Efficacy in Controlling Human Pathogens
Barbara Smal, University of Guelph; Azadeh Namvar, University of Guelph; Bengt Schumacher, OMAF; Keith Warriner, University of Guelph
P1-97
Growth of Escherichia coli O157: H7 and O104: H4 during Sprouting and Microgreen Production from Inoculated Radish Seeds
Zhenlei Xiao, University of Maryland; Xiangwu Nou, U.S. Department of Agriculture-ARS; Yaguang Luo, U.S. Department of Agriculture-ARS; Qin Wang, University of Maryland
P1-98
Comparison of the Efficacy of Three Organic Sanitizers with 20,000 ppm Calcium Hypochlorite for Inactivation of Salmonella on Artificially Contaminated Alfalfa Seeds
Qing Li, Illinois Institute of Technology; Shuoqiu Song, Illinois Institute of Technology; Haijing Shen, Illinois Institute of Technology; Tong-Jen Fu, U.S. Food and Drug Administration
P1-99
Survival and Growth of Salmonella during Sprouting of Alfalfa Seeds as Affected by Seed Treatment with 20,000 ppm Calcium Hypochlorite and Sprouting Conditions
Shuoqiu Song, Illinois Institute of Technology; Jing Xie, Illinois Institute of Technology; Qing Li, Illinois Institute of Technology; Lan Wei, Illinois Institute of Technology; Tong-Jen Fu, U.S. Food and Drug Administration
P1-100
Inactivation of Human Norovirus and Its Surrogates on Alfalfa Seeds by Aqueous Ozone
Qing Wang, University of Delaware; Sarah Markland, University of Delaware; Kalmia Kniel, University of Delaware
P1-101
Attachment and Biofilm Formation by Selected Strains of Salmonella enterica and Escherichia coli of Fresh Produce Origin
Ran Han, The University of Georgia; Yaa Asantewaa Klu, The University of Georgia; Jinru Chen, The University of Georgia
P1-102
Effectiveness of Chlorine Dioxide Gas Treatment in Inactivating Salmonella enterica on Mungbean Sprouts
Bassam Annous, U.S. Department of Agriculture-ARS-ERRC; Vara Prodduk, Rutgers, The State University of New Jersey; LinShu Liu, U.S. Department of Agriculture-ARS-ERRC; Kit Keith Yam, Rutgers, The State University of New Jersey
P1-103
Inactivation of Salmonella enterica on Artificially Inoculated Mature Green Tomatoes: Efficacy and Cost Modeling of Gaseous Chlorine Dioxide
Bassam Annous, U.S. Department of Agriculture-ARS-ERRC; Joseph Sites, U.S. Department of Agriculture-ARS-ERRC; Angela Burke, U.S. Department of Agriculture-ARS-ERRC
P1-104
Prevention of the Internalization of Salmonella spp. in Growing Mung Bean Sprouts with the Vegetable-associated Bacillus subtilis Strain LCA1
Zhenyu Shen, University of Missouri-Columbia; Azlin Mustapha, University of Missouri-Columbia; Mengshi Lin, University of Missouri-Columbia; Daidong Deng, Lincoln University; Guolu Zheng, Lincoln University
P1-105
Comparison of the Efficacy of Various Sanitizers and Hot Water Treatment in Inactivating Escherichia coli O157:H7, Listeria monocytogenes, Salmonella spp., and Natural Microflora on Mung Bean Sprouts
Li Kai Phua, National University of Singapore; Shan Yu Neo, Agri-Food & Veterinary Authority of Singapore; Gek Hoon Khoo, Agri-Food & Veterinary Authority of Singapore; Hyun-Jung Chung, Inha University; Hyun-Gyun Yuk, National University of Singapore
P1-106
Fate of Listeria monocytogenes on Freshly Harvested Georgia-grown Cantaloupes Treated with Sanitizers
Cathy Webb, University of Georgia; Marilyn Erickson, University of Georgia; Michael Doyle, University of Georgia
P1-107
Microbiological Evaluation of Florida Cantaloupe Packinghouses
Loretta Friedrich, University of Florida; Michelle Danyluk, University of Florida; Alicia Whidden, Hillsborough County Extension Service; Laura Strawn, Cornell University; Martin Wiedmann, Cornell University; Martha Robert, University of Florida
P1-108
Efficacy of Household Sanitizers for the Reduction of Listeria monocytogenes and Salmonella s.v. Typhimurium on Cantaloupe
Jun Won Chang, Purdue University; Amanda Deering, Purdue University; Robert Pruitt, Purdue University
P1-109
Control of the Microbial Quality of Cantaloupes via Employment of a Processing Line Incorporating Chlorine Dioxide as an Intervention Step
Jeffrey Chandler, University of Wyoming; Alma Perez-Mendez, Colorado State University; Shannon Coleman, Colorado State University; Wanda Manley, Wyoming Public Health Laboratory; Michelle Danyluk, University of Florida; Marisa Bunning, Colorado State University; Lawrence Goodridge, Colorado State University; Bledar Bisha, University of Wyoming
P1-110
Quantitative Transfer of Listeria monocytogenes during Mechanical Slicing and Dicing of Cantaloupe and Honeydew Melon
Rocky Patil, Michigan State University; Jake Thorns, Michigan State University; Elliot Ryser, Michigan State University
P1-111
A Surveillance Study of Fresh Cantaloupe from Indiana Farms Following 2012 Salmonella Outbreak
Hesham Elgaali, Indiana State Department of Health; Laurie Kidwell, Indiana State Department of Health; Zhao Xiaomin, Indiana State Department of Health; Mark Forster, Indiana State Department of Health
P1-112
Control of Salmonella Cross-contamination between Intact Green Round Tomatoes in a Model Flume
Scott Gereffi, University of Florida; Keith Schneider, University of Florida
P1-113
Efficacy of Four Different Sanitizer Treatments against Salmonella on Smooth and Interlocking Belts during Conveyance of Diced Tomato
Haiqiang Wang, Michigan State University; Elliot Ryser, Michigan State University
P1-114
Effect of Cooling Methods, pH and Temperature on Salmonella Survival on Inoculated Intact and Pureed Strawberries and Pureed Tomatoes
Aswathy Sreedharan, University of Florida; Oleksandr Tokarsky, I. Ya. Horbachevsky Ternopil State Medical University; Steven Sargent, University of Florida; Keith Schneider, University of Florida
P1-115
Influence of Temperature Differential between Green Tomatoes and Postharvest Water on Salmonella Internalization
Ashley Turner, University of Florida; Michelle Danyluk, University of Florida
P1-116
Salmonella Newport and Typhimurium Colonization of Fruit Differs from Leaves in Various Tomato Cultivars
Sanghyun Han, University of Maryland; Shirley Micallef, University of Maryland
P1-117
Environmental Reservoirs of Salmonella spp. in Field and Water Samples Associated with North Carolina Tomato Production
Diane Ducharme, North Carolina State University; Christopher Gunter, North Carolina State University; Penelope Perkins-Veazie, North Carolina State University; Otto Simmons, North Carolina State University; Lee-Ann Jaykus, North Carolina State University; Jie Zheng, U.S. Food and Drug Administration-CFSAN; Insook Son, U.S. Food and Drug Administration-CFSAN; Eric Brown, U.S. Food and Drug Administration-CFSAN; Rebecca Bell, U.S. Food and Drug Administration-CFSAN
P1-118
Evaluation of Risk Factors Affecting Transmission and Persistence of Salmonella spp. in Hydroponically Grown Tomatoes
Shannon Coleman, Colorado State University; Bledar Bisha, University of Wyoming; Kelly Blume, Colorado State University; Vanessa Sandoval, Colorado State University; Nicholas Drury, Colorado State University; Holly Kesseler, Colorado State University; Alma Perez-Mendez, Colorado State University; Jeffrey Chandler, University of Wyoming; Ben Franz, Colorado State University; Ryan Jaebker, Colorado State University; Donald Schaffner, Rutgers, The State University of New Jersey; Jeffrey LeJeune, The Ohio State University; Marisa Bunning, Colorado State University; Steven Newman, Colorado State University; Lawrence Goodridge, Colorado State University
P1-119
Evaluation of a Most Probable Number Technique for the Simultaneous Quantification of Salmonella spp., Shigella spp. and Listeria monocytogenes in Saladette Tomatoes (Lycopersicon esculentum Mill) and Serrano Peppers
Jorge Sánchez-Camarena, Universidad de Guadalajara; Erika Navarro-Trigueros, Universidad de Guadalajara; Christian Hernández-Silva, Universidad de Guadalajara; Elisa Cabrera-Díaz, Universidad de Guadalajara; Julia Pérez-Montaño, Universidad de Guadalajara; Luz Eduviges Garay-Martínez, Universidad de Guadalajara; Montserrat Hernández-Iturriaga, Universidad Autónoma de Querétaro; Nanci Edid Martínez-Gonzáles, Universidad de Guadalajara
P1-120
Survival of Virulent and Mutated Salmonella enterica Newport and Typhimurium Strains on Tomato Plants and in Soils
Ganyu Gu, Virginia Tech; Lily Yang, Virginia Tech; Renee Boyer, Virginia Tech; Robert Williams, Virginia Tech; Steven Rideout, Virginia Tech
P1-121
Examination of Overhead and Drip Irrigation and Chlorine Dioxide Treatment of Irrigation Water
Karen Killinger, Washington State University; Achyut Adhikari, Louisiana State University; Craig Cogger, Washington State University; Andy Bary, Washington State University
P1-122
Application of Pulsed Light (PL) to Decontaminate Escherichia coli O157:H7 and Salmonella and Preserve Quality on Raw Raspberries
Wenqing Xu, University of Delaware; Haiqiang Chen, University of Delaware; Changqing Wu, University of Delaware
P1-123
Variables Affecting Virus Survival in Strawberries under High Hydrostatic Pressure
Hao Pan, Illinois Institute of Technology; Eduardo Patazca, Illinois Institute of Technology; Matthew Buenconsejo, Illinois Institute of Technology; Carol Shieh, U.S. Food and Drug Administration-IFSH
P1-124
Norovirus Binding to Berries May be Independent of the Presence of HBGA-like Moieties and is Potentially Related to Interactions with Natural Microflora
Jonathan Baugher, North Carolina State University; Rebecca Goulter, North Carolina State University; Lee-Ann Jaykus, North Carolina State University
P1-125
Persistence of Escherichia coli on Basil in Tropical Environments
Michael Melzer, University of Hawaii; Anne-Laure Moyne, University of California-Davis; Tyann Blessington, Oak Ridge Institute for Science and Education; Anne Alvarez, University of Hawaii; Michele Jay-Russell, University of California-Davis; Linda Harris, University of California-Davis
P1-126
Survival of Escherichia coli in Field-inoculated Basil
Anne-Laure Moyne, University of California-Davis; Linda Harris, University of California-Davis
P1-127
Efficacy of Elution Solutions to Detect Cyclospora cayetanensis, Cryptosporidium parvum, and Toxoplasma gondii from Basil
Venessa Chandra, University of Georgia; Maria Torres, University of Georgia; Ynes Ortega, University of Georgia
P1-129
Irrigation, Manure, and Soil Type Influences on Survival and Persistence of Non-pathogenic E. coli and E. coli O157:H7 in a Greenhouse Environment
Celia Whyte, University of Maryland Eastern Shore; Corrie Cotton, University of Maryland Eastern Shore; Fawzy Hashem, University of Maryland Eastern Shore; Manan Sharma, U.S. Department of Agriculture-ARS; Patricia Millner, U.S. Department of Agriculture-ARS
P1-130
Survival of Escherichia coli and Attenuated E. coli O157:H7 in Manure-amended Soils in the Delmarva Peninsula as Influenced by Winter Weather Conditions
Corrie Cotton, University of Maryland Eastern Shore; Fawzy Hashem, University of Maryland Eastern Shore; Manan Sharma, U.S. Department of Agriculture-ARS; Patricia Millner, U.S. Department of Agriculture-ARS
P1-131
The Effect of Manure Application Method on the Persistence of Eschericha coli in Manure-amended Soils in Southeastern Pennsylvania
Lorna Graham, University of Maryland Eastern Shore; Daniel Wright, University of Maryland Eastern Shore; Fawzy Hashem, University of Maryland Eastern Shore; Corrie Cotton, University of Maryland Eastern Shore; Alyssa Collins, Pennsylvania State University; Kathryn White, U.S. Department of Agriculture-ARS; Richard Stonebraker, U.S. Department of Agriculture-ARS; Manan Sharma, U.S. Department of Agriculture-ARS; Patricia Millner, U.S. Department of Agriculture-ARS
P1-132
Evaluation of Non-pathogenic Escherichia coli Isolates to be Used in Field Trials Examining the Persistence of Pathogens in Untreated Soil Amendments
Daniel Wright, University of Maryland Eastern Shore; Natalia Macarisin, U.S. Department of Agriculture-ARS; Eric Handy, U.S. Department of Agriculture-ARS; Lorna Graham, University of Maryland Eastern Shore; Trevor Suslow, University of CA; Fawzy Hashem, University of Maryland Eastern Shore; Patricia Millner, U.S. Department of Agriculture-ARS; Manan Sharma, U.S. Department of Agriculture-ARS
P1-133
Efficacy of Two Hand-hygiene Methods to Reduce Organic Matter and Fecal Contamination on Farmworker Hands during Harvest
Norma Heredia, Universidad Autonoma de Nuevo Leon; Anna Fabiszewski de Aceituno, Emory University; Alexandra Stern, Emory University; Lee-Ann Jaykus, North Carolina State University; Jennifer Gentry-Shields, North Carolina State University; Juan Leon, Emory University; Faith Bartz, Emory University; Santos Garcia, Universidad Autonoma de Nuevo Leon
P1-134
A Longitudinal Comparison of Eschericha coli, Salmonella and Enteric Viruses on Wildlife Feces to Evaluate Potential Microbial Niches
Patrick Spanninger, University of Delaware; Rafael Castaneda, University of Delaware; Beth Pantuliano, University of Delaware; Kalmia Kniel, University of Delaware
P1-135
Investigating the Effect of Salmonella Biocontrol Agent Paenibacillus alvei and Poultry Litter Soil Amendment on the Eastern Shore Tomato Microbiome
Sarah Allard, University of Maryland; Anna Wallis, University of Maryland; Elizabeth Prinkey, University of Maryland; James Pettengill, U.S. Food and Drug Administration; Jie Zheng, U.S. Food and Drug Administration-CFSAN; Andrea Ottesen, U.S. Food and Drug Administration; Christopher Walsh, University of Maryland; Eric Brown, U.S. Food and Drug Administration-CFSAN; Peter Evans, U.S. Food and Drug Administration; Shirley Micallef, University of Maryland
P1-136
Isolation and Characterization of Salmonella from North Florida Surface Waters
Travis Chapin, University of Florida; Gabriel Mootian, Rutgers University; Rachel McEgan, University of Florida; Sweeya Reddy, University of Florida; Loretta Friedrich, University of Florida; Jeffrey Chandler, University of Wyoming; Lawrence Goodridge, Colorado State University; Michelle Danyluk, University of Florida; Keith Schneider, University of Florida; Donald Schaffner, Rutgers, The State University of New Jersey
P1-137
Microbial Quality of Surface Agricultural Water in Central Florida
Zeynal Topalcengiz, University of Florida; Michelle Danyluk, University of Florida
P1-138
Low Concentration of Salmonella in Farm Ponds and Irrigation Distribution Systems Used for Mixed Produce Production in Southern Georgia
Michele Jay-Russell, University of California-Davis; George Vellidis, University of Georgia; Debbie Coker, University of Georgia; Herman Henry, University of Georgia; Peiman Aminabadi, University of California-Davis; Elizabeth Antaki, University of California-Davis
P1-139
The Occurrence of Bacterial and Viral Foodborne Pathogens in Irrigation Waters in Southern Arizona
Dusty Tyree, The University of Arizona; Jason Torrey, The University of Arizona; Jonathan Sexton, The University of Arizona; Rachael Zinn, The University of Arizona; Masaaki Kitajima, The University of Arizona; Kelly Bright, The University of Arizona
P1-140
Evaluation of Indicator Escherichia coli, Fecal Coliforms, E. coli O157 and Salmonella spp. in Surface Waters of the Southwest Desert Canal Network
Ronald Bond, University of California-Davis; Melissa Partyka, University of California-Davis; Peiman Aminabadi, University of California-Davis; Channah Rock, University of Arizona; Kurt Nolte, University of Arizona; Edward Atwill, University of California-Davis; Michele Jay-Russell, University of California-Davis
P1-141
Examination of Indicator Organism Levels in Open Surface Water Sources Used for Irrigation and Overhead Cooling of Apples in Washington
Karen Killinger, Washington State University; Kim Thayer, Washington State University; Jonathan Nagata, University of Washington; John Meschke, University of Washington; Ines Hanrahan, Washington Tree Fruit Research Commission
P1-142
Prevalence of Shiga Toxin-producing Escherichia coli in Irrigation Waters and Fresh Produce in British Columbia, Canada
Stephanie Nadya, University of British Columbia; Kevin Allen, University of British Columbia; Kim Ziebell, Public Health Agency of Canada; Roger Johnson, Public Health Agency of Canada; Susan Bach, Agriculture and Agri-Food Canada; Pascal Delaquis, Agriculture and Agri-Food Canada
P1-143
Role of Washing Water Parameters on Inactivation of Salmonella during the Disinfection Step of Minimally Processed Vegetables
Daniele Fernanda Maffei, University of Sao Pãulo; Flavia Cristina Braga, University of Sao Pãulo; Rubia de Souza Olivo, University of Sao Pãulo; Anderson de Souza Sant’Ana, University of Campinas; Bernadette D.G.M. Franco, University of Sao Pãulo
P1-144
The Effect of Water Velocity on Escherichia coli O157:H7 Transfer from Inoculated Lettuce to Wash Water in a Closed Pipe System
Lin Ren, Michigan State University; Beatriz Mazón, Michigan State University; Bradley Marks, Michigan State University; Elliot Ryser, Michigan State University
P1-145
The Effect of Water Hardness on the Efficacy of Sodium Hypochlorite Inactivation of Eschericha coli O157:H7 in Water
Sara Swanson, Illinois Institute of Technology; Christina Megalis, U.S. Food and Drug Administration; David Laird, U.S. Food and Drug Administration-USPHS; Tong-Jen Fu, U.S. Food and Drug Administration
P1-146
Efficacy of Neutral Electrolyzed Water in Reducing Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 on Fresh Produce Items Using Modified Washing Procedures and Simulated Food Service Operation Conditions
George Afari, University of Georgia; Yen-Con Hung, University of Georgia; C. Harold King, Chick-fil-A, Inc.; Ravirajsinh Jadeja, University of Georgia
P1-147
A Multi-step Screening Procedure for Selecting Surrogate Organisms for Use in Validating Fresh Produce Washing Processes
Arlette Shazer, U.S. Food and Drug Administration; Diana Stewart, U.S. Food and Drug Administration; Kaiping Deng, U.S. Food and Drug Administration; Mary Lou Tortorello, U.S. Food and Drug Administration
P1-149
Microbial Profiles of Selected Fresh Produce from Farmers’ Markets and Retail Stores in Illinois
Hee Kyung Park, University of Illinois at Urbana-Champaign; Hao Feng, University of Illinois at Urbana-Champaign; James Theuri, University of Illinois Extension
P1-150
Microbial Safety of Fresh Herbs from Los Angeles, Orange County, and Seattle Farmers’ Markets
Donna Levy, Chapman University; Nicola Beck, University of Washington; Alexandra Kossik, University of Washington; Taylor Patti, Chapman University; John Meschke, University of Washington; Melissa Calicchia, Food Safety Solutions; Rosalee Hellberg, Chapman University
P1-151
Prevalence of Salmonella, Escherichia coli O157:H7 and Shigella in Selected Fresh Produce from Supermarkets, Local Markets and Farmers’ Markets
Yi Su, University of Florida; Wei-Yea Hsu, University of Florida; Amarat Simonne, University of Florida; Tung-Shi Huang, Auburn University
P1-152
Laboratory and Industrial-scale Examination of Post-harvest Chlorine and Chlorine Dioxide Antimicrobial Applications for Whole, Fresh Gala Apples
Karen Killinger, Washington State University; Kim Thayer, Washington State University; Molly Mayer, Washington State University; Richard Dougherty, Washington State University; Ines Hanrahan, Washington Tree Fruit Research Commission
P1-153
Survival of Listeria monocytogenes and Salmonella spp. on the Epicarp of Avocados (Persea americana var. Hass) as Affected by Simulated Transport Conditions
Liliana Martínez-Chavez, Universidad de Guadalajara; Sandra Ávila-Delgado, Universidad de Guadalajara; Jacob Gómez-Rivera, Universidad de Guadalajara; Nanci Edid Martínez-Gonzáles, Universidad de Guadalajara; Ofelia Rodriguez-Garcia, Universidad de Guadalajara; Jeannette Barba, Universidad de Guadalajara; Alejandro Castillo, Texas A&M University; Elisa Cabrera-Díaz, Universidad de Guadalajara
P1-154
Efficacy of Peroxyacetic Acid against Salmonella and Native Microbiota in Float Tank Water Used during Commercial Walnut Hulling
Gordon Davidson, University of California-Davis; Vanessa Lieberman, University of California-Davis; Linda Harris, University of California-Davis
P1-155
Preliminary Survey of the Occurence of Foodborne Human Pathogens in Pecan Production Fields
Shefali Dobhal, Oklahoma State University; Charles Rohla, The Samuel Roberts Noble Foundation; Mike Smith, Oklahoma State University; Guodong Zhang, U.S. Food and Drug Administration; Chris Timmons, Oklahoma State University; Li Ma, Oklahoma State University
P1-156
Prediction of Listeria monocytogenes Presence in a Frozen-vegetable Processing Plant Using Traditional Microbial Indicators and Listeria spp
Angélica Godínez-Oviedo, Universidad Autónoma de Querétaro; Dulce Avila-Vega, Universidad Autónoma de Querétaro; M. Carmen González-López, Universidad Autónoma de Querétaro; Gerardo Nava, Universidad Autónoma de Querétaro; Montserrat Hernández-Iturriaga, Universidad Autónoma de Querétaro
P1-157
Emergence of Carbapenem-resistant Enterobacteraceae: From Healthcare to Harvest
Erika Johnson, North Carolina A&T State University; Leonard Williams, North Carolina A&T State University; Janak Khatiwada, North Carolina A&T State University; Decima Washington, North Carolina A&T State University; Dustin Smith, North Carolina A&T State University; Shurrita Davis, North Carolina AT&T University
P1-158
Effects of Physical Variables on Salmonella Transfer from Produce to Stainless Steel
Beatriz Mazón, Michigan State University; Bradley Marks, Michigan State University; Lin Ren, Michigan State University; Elliot Ryser, Michigan State University
P1-159
Effect of Sanitizer and in-package Atmosphere on Listeria monocytogenes Growth in Diced Yellow Onions
Andrew Scollon, Michigan State University; Natalie Page, Michigan State University; Eva Almenar, Michigan State University; Elliot Ryser, Michigan State University
P1-160
Rapid Detection and Quantification of Salmonella enterica in Peanut Butter by a rRNA Detection System
Shyam Verma, Sigma-Aldrich Co.; Jvo Siegrist, Sigma-Aldrich Co.; Kunal Rehani, Sigma-Aldrich Co.
P1-161
Determination of the Dry Heat-resistance of Salmonella spp. and Eschericha coli O157:H7 Dried on to Stainless Steel Surfaces in Low Loisture Food Production Environments
Rob Limburn, Campden BRI; Joy Gaze, Campden BRI; Ryan Busby, Campden BRI; Roy Betts, Campden BRI
P1-162
Impact of Inoculation Procedures on Thermal Resistance of Salmonella in Wheat Flour and Associated Repeatability of Results
Ian Hildebrandt, Michigan State University; Bradley Marks, Michigan State University; Elliot Ryser, Michigan State University; Rossana Villa-Rojas, Washington State University; Juming Tang, Washington State University; Sarah Buchholz, Michigan State University
P1-163
Improving Safety of Wheat Milled Products through Processing
Luis Sabillon, University of Nebraska-Lincoln; Andreia Bianchini, University of Nebraska-Lincoln; Jayne Stratton, University of Nebraska-Lincoln; Devin Rose, University of Nebraska-Lincoln; Rolando Flores, University of Nebraska-Lincoln
P1-164
Decline of Salmonella on Artificially Contaminated Dry Pet Food
Elisabetta Lambertini, University of Maryland; Huilin Cao, University of Maryland; Miao Guo, University of Maryland; Abhinav Mishra, University of Maryland; Robert Buchanan, University of Maryland; Abani Pradhan, University of Maryland
P1-165
Influence of Sucrose Content on the Survival of Salmonella in Low-water Activity Whey Protein Model Systems at 70°C
Stephanie Barnes, University of Georgia; Joseph Frank, University of Georgia
P1-166
Dry Talc Inoculum Preparation Procedure with Salmonella and the Surrogate (Enterococcus faecium) for Challenge Studies in Low-moisture Foods
Elena Enache, Grocery Manufacturers Association; Ai Kataoka, Grocery Manufacturers Association; Glenn Black, Grocery Manufacturers Association; Carla Napier, Grocery Manufacturers Association; Richard Podolak, Grocery Manufacturers Association; Melinda Hayman, Grocery Manufacturers Association
P1-167
Thermal Resistance of Salmonella Tennessee, Salmonella Typhimurium DT104 and Enterococcus faecium in Peanut Paste Formulations at Two Levels of Water Activity (0.3 and 0.6) and Fat Concentrations (47 and 56%) at 75°C
Elena Enache, Grocery Manufacturers Association; Ai Kataoka, Grocery Manufacturers Association; Glenn Black, Grocery Manufacturers Association; Richard Podolak, Grocery Manufacturers Association; Philip Elliott, Grocery Manufacturers Association; Richard Whiting, Exponent, Inc.; Melinda Hayman, Grocery Manufacturers Association
P1-168
Effect of Hot Air Roasting Process on the Survival of Salmonella spp. and Listeria monocytogenes during Sunflower Seeds Processing
Balasubrahmanyam Kottapalli, ConAgra Foods, Inc.; Denise Becker, ConAgra Foods, Inc.; Yanyan Huang, ConAgra Foods, Inc.; Michael Meyer, ConAgra Foods, Inc.; Stefanie Gilbreth, ConAgra Foods, Inc.; Steven Hermansky, ConAgra Foods, Inc.
P1-169
Thermal Resistance of Salmonella during Brewing Related to Length of Storage of Inoculated Green Tea Leaves
Susanne Keller, U.S. Food and Drug Administration-IFSH; Christina Stam, U.S. Food and Drug Administration; Dana Gradl, U.S. Food and Drug Administration-IFSH; Shannon Pickens, Illinois Institute of Technology
P1-170
Impact of Refrigerated Storage on Thermal Inactivation of Enterococcus faecium NRRL B-2354 and Salmonella Enteritidis PT 30 ATCC BAA-1045 on Inoculated Almonds
Chris Theofel, University of California-Davis; Linda Harris, University of California-Davis
P1-171
Determining Validity of Enterococcus Faecium as Surrogate for Salmonella under Stagnant Dry Heating of Peanuts
Pardeepinder Brar, University of Florida; Michelle Danyluk, University of Florida
P1-172
Effect of Inoculation Method on the Survival of Salmonella Tennessee on Whole Black Peppercorns
Lauren Bowman, Virginia Tech; Monica Ponder, Virginia Tech; Robert Williams, Virginia Tech; Kumar Mallikarjunan, Virginia Tech
P1-173
The Influence of Fat Content on the Survival of Salmonella in a Low-water Activity Model Food System at 70°C
Lisa Trimble, University of Georgia; Joseph Frank, University of Georgia
P1-174
Effect of Almond Product Structure on X-ray Inactivation Kinetics of Salmonella
Sanghyup Jeong, Michigan State University; Bradley Marks, Michigan State University; Elliot Ryser, Michigan State University
P1-175
Effect of Product Structure on Thermal Resistance of Salmonella Enteritidis PT30 on Whole Almonds, in Almond Meal and in Almond Butter
Pichamon Limcharoenchat, Michigan State University; Bradley Marks, Michigan State University; Ian Hildebrandt, Michigan State University; Nicole Hall, Michigan State University
P1-176
Inactivation of Salmonella in Low-moisture Products at Relatively High Temperatures Using Radiofrequency-assisted Heat Treatments (RFHT)
Rossana Villa-Rojas, Washington State University; Juming Tang, Washington State University; Mei-Jun Zhu, Washington State University; Shuxiang Liu, Washington State University; Ravi Tadapaneni, Washington State University; Roopesh Syamaladevi, Washington State University; Bradley Marks, Michigan State University
P1-177
Efficacy of Dry and Moist Heat on the Inactivation of Salmonella in a Low-moisture Powder Residue Attached to Stainless Steel Surfaces
Michael James, Michigan State University; Matthew Walch, Michigan State University; Erdal Tuncan, Dupont Nutrition and Health; Tamrat Belete, Dupont Nutrition and Health; Sanghyup Jeong, Michigan State University; Bradley Marks, Michigan State University
P1-178
Survival of Salmonella on Dried Fruits and in Aqueous Dried Fruit Homogenates as Affected by Temperature
David Mann, University of Georgia; Larry Beuchat, University of Georgia
P1-179
Effect of Rapid Desiccation on Thermal Resistance of Salmonella in Wheat Flour
Danielle Smith, Michigan State University; Bradley Marks, Michigan State University
P1-180
Salmonella Biofilm Formation on Peppercorns and Polystyrene
Nathan Briggs, Virginia Tech; Robert Williams, Virginia Tech; Monica Ponder, Virginia Tech; Lauren Bowman, Virginia Tech; Kumar Mallikarjunan, Virginia Tech
P1-181
Thermal Resistance of Salmonella in Low-moisture Foods
Elizabeth Grasso, Illinois Institute of Technology; Gregory Fleischman, U.S. Food and Drug Administration; Nathan Anderson, U.S. Food and Drug Administration/IFSH
P1-182
Apple, Orange, and Strawberry Juices Treated with Pulsed Light (PL). Effect on Some Inoculated Microorganisms and Native Flora during Refrigerated Storage
Mariana Ferrario, Buenos Aires University; Stella Maris Alzamora, Buenos Aires University; Sandra Guerrero, Buenos Aires University
P1-183
Study of the Inactivation of Saccharomyces cerevisiae in Apple Juice by High Intensity Pulsed Light (PL) and Ultrasound (US): Assessment of the Physiological Status by Flow Cytometry (FC) and Transmission Electron Microscopy (TEM)
Mariana Ferrario, Buenos Aires University; Stella Maris Alzamora, Buenos Aires University; Sandra Guerrero, Buenos Aires University
P1-184
Evaluation of a Rapid Approach for the Detection and Direct Identification of Spoilage Microorganisms in Fruit Juices
Patricia Rule, bioMérieux; Carolyn Beres, bioMérieux; Hari Prakash Dwivedi, bioMérieux; Gregory Devulder, bioMérieux; David Pincus, bioMérieux
P1-185
Search for a Natural Intervention against Listeria monocytogenes in Wheatgrass Juice
Angela Shaw, Iowa State University; Amanda Svoboda, Iowa State University; Stephanie Jung, Iowa State University
P1-186
Selection of Autochthonous Saccharomyces Yeasts from Queretaro Vineyards Based on Safety and Enological Qualities
Dalia Elizabeth Miranda-Castilleja, Universidad Autónoma de Querétaro; Eunice Ortiz-Barrera, Universidad Autónoma de Querétaro; Sofia Arvizu-Medrano, Universidad Autónoma de Querétaro; Ramón Álvar Martínez-Peniche, Universidad Autónoma de Querétaro
P1-187
Development of a Real-time PCR Assay for the Detection of the Spoilage Yeast Zygosaccharomyces bailii in Wine and Fruit Juices
Julie Maupeu, Université de Bordeaux; Sophie Pierre, Bio-Rad Laboratories; Cecile Oger-Duroy, Bio-Rad Laboratories; Jean-Francois Mouscadet, Bio-Rad Laboratories
P1-188
New Chromogenic Media for Rapid Detection of Clostridium perfringens
Shyam Verma, Sigma-Aldrich Co.; Jvo Siegrist, Sigma-Aldrich Co.; Mohammad Manafi, Medical University of Vienna
P1-189
Detection of Viable Escherichia coli in Environmental Water Using a Combined Propidium Monoazide Staining-real-time PCR
Yuan Yuan, University of Missouri-Columbia
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