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P2-96 Evaluation of the 3M™ Petrifilm™ Salmonella Express System for the Detection of Salmonella in Select Foods: Collaborative Study

Tuesday, August 5, 2014
Exhibit Hall D (Indiana Convention Center)
Erin Crowley, Q Laboratories, Inc., Cincinnati, OH
Patrick Bird, Q Laboratories, Inc., Cincinnati, OH
Jonathan Flannery, Q Laboratories, Inc., Cincinnati, OH
Kiel Fisher, Q Laboratories, Inc., Cincinnati, OH
Benjamin Bastin, Q Laboratories, Inc., Cincinnati, OH
Kateland Koch, Q Laboratories, Inc., Cincinnati, OH
William Judd, Q Laboratories, Inc., Cincinnati, OH
James Agin, Q Laboratories, Inc., Cincinnati, OH
David Goins, Q Laboratories, Inc., Cincinnati, OH
Robert Jechorek, 3M Food Safety, St. Paul, MN
Introduction: The 3M™ Petrifilm™ Salmonella Express System (SALX) is a simple, ready-to-use chromogenic culture medium system for the rapid qualitative detection and biochemical confirmation of Salmonella spp. in food and environmental samples.  This new method utilizes the  proprietary  3M™ Salmonella Enrichment Base and 3M™ Salmonella Enrichment Supplement which allows for presumptive results in as little as 40 hours from low microbial background foods (<104 CFU/g) and 48 hours from high microbial background foods (≥104).  

Purpose: The purpose of this AOAC OMA Collaborative Study was to compare the new method to the FDA/BAM for dry dog food (375g) and the USDA/FSIS-MLG for raw ground beef (25g).

Methods:   This new method was compared in a multilaboratory collaborative study to the FDA/BAM Chapter 5 method and the USDA MLG 4.06 reference methods.  A total of 17 laboratories located within the continental U.S participated.  Each matrix was artificially contaminated with Salmonella at 3 inoculation levels: a low-level inoculum of 0.2-2 CFU/test portion, a high-level inoculum 2-5 CFU/test portion and an un-inoculated control level 0 CFU/test portion.  All test portions were confirmed via traditional confirmation procedures and an alternative confirmation procedure directly from the 3M™ Petrifilm SALX Plate.

Results: In this study, over 500 samples were analyzed by the new method and the reference methods for both the raw ground beef and dry dog food.  Statistical analysis was conducted according to the Probability of Detection (POD) statistical model.  No statistically significant difference was observed between the new and reference methods for both matrices.  Additionally, no differences were observed between the alternative and traditional confirmation procedures for the new method. 

Significance: The collaborative study demonstrated the reliability and sensitivity of the 3M™ Petrifilm Salmonella Express System for the rapid detection of Salmonella in food products using both 25-g and 375-g sample sizes.

Back to: Poster Session - Pathogens, Epidemiology, Novel Laboratory Methods, Food Defense, Communication Outreach and Education, General Microbiology, Dairy and Other Food Commodities, Food Toxicology
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