Purpose: Determine the antimicrobial efficacy of fulvic acid against E. coli O157:H7 in organic leafy greens at refrigerated temperatures.
Methods: Organic baby and adult spinach, and romaine and iceberg lettuce were inoculated with a cocktail of three E. coli O157:H7 strains (106 CFU/ml). Fulvic acid I, II, III and IV at different concentrations (1, 2 and 3%) were prepared in sterile deionized water. The inoculated greens were washed in each treatment solution for 2 minutes, bagged and refrigerated at 4°C. Hydrogen peroxide and water were used as the controls. Surviving bacterial populations were enumerated on days 0, 1, and 3 of storage.
Results: Significant log reductions (P < 0.01) were observed in pathogen populations on leafy greens treated with all fulvic acid concentrations. Fulvic acid III was most effective, with a 3-log reduction for each leafy green by day 3. Fulvic acid II (3%) and III (1%) showed a 2.8 and 2.5-log CFU/g reduction, respectively, in romaine lettuce and 1.6 to 2.0-log reduction for the rest of the leafy greens. Fulvic acid I and IV resulted in 1.4 to 1.8-log CFU/g reductions, respectively.
Significance: Fulvic acid is an effective natural antimicrobial and can be used as a wash treatment for organic leafy greens stored at refrigerated temperatures.