Purpose: This research was done to evaluate which were the steps that promote the major microbiological contamination during the processing.
Methods: The heart of palm process of a big processing industry had the following steps: I) receipt of the heart of palm; II) removing of the sheaths; III) cutting or picking the shoots; IV) washing; V) arranging in bottle; VI) adding solution; VII) exhausting; VIII) sealing; IX) pasteurization; X) storage. Using specific Petrifilm from 3M, the following microbiological analyses were done after steps III; V; and IX: aerobic mesophilic bacteria, fecal coliform, Staphylococcus spp., mold and yeast.
Results: Initial population (step III) was: 4.7 x 108 CFU/g for aerobic mesophilic bacteria; 1.2 x 105 CFU/g for fecal coliforms; 2.9 x 105 CFU/g for Staphylococcus spp.; 5.9 x 105 CFU/g for mold and yeast. After step V: 7.7 x 106 CFU/g for aerobic mesophilic bacteria; 2.6 x 105 CFU/g for fecal coliforms; 1.2 x 104 CFU/g for Staphylococcus spp.; 2.2 x 104 CFU/g for mold and yeast. The reductions observed were obtained due the washing step. After pasteurization the population observed was: 2.9 x 103 CFU/g for aerobic mesophilic bacteria; none fecal coliforms; 3.2 x 102 CFU/g for Staphylococcus spp.; 2.3 x 103 CFU/g for mold and yeast.
Significance: The results suggest that some improvement must be done during steps I and II to ensure a lower microorganism level in the other steps of the processing.