Monday, August 4, 2014
Exhibit Hall D (Indiana Convention Center)
Erika Johnson, North Carolina A&T State University, Kannapolis, NC
Leonard Williams, North Carolina A&T State University, Kannapolis, NC
Janak Khatiwada, North Carolina A&T State University, Kannapolis, NC
Decima Washington, North Carolina A&T State University, Kannapolis, NC
Dustin Smith, North Carolina A&T State University, Kannapolis, NC
Shurrita Davis, North Carolina AT&T University, Kannapolis, NC
Introduction: The emerging carbapenemase group classified as Amber Class B Metallo-β-Lactamases (MBL’s) has been an increasing concern worldwide. Recent reports of
Klebsiella pneumonia producing IMP, VIM, and NDM types have heightened interest over additional transmissible carbapenem- resistance in
Enterobacteriaceae. Carbapenem-resistant
Enterobacteriaceae (CRE) is primarily associated with the healthcare field but now is becoming a food safety issue.
Purpose: The objective of this study was to isolate and identify Metallo-β-Lactamases producing Staphylococcus aureus from porcine and produce samples.
Methods: The 127 samples tested were comprised of 50 porcine and 77 produce samples. The strains were isolated and identified according to the procedure of the Bacteriological Analytical Manual with some modification to identify the presence of Staphylococcus aureus. DNA was collected using PrepSEQ Rapid Spin Sample Preparation Kit in accordance with manufacturer’s instructions and then Polymerase Chain Reaction (PCR) was performed.
Results: Results demonstrated that 5.2 % of samples collected produced NDM, 23.4% produced VIM, 18.2% produced SPM-1, and 15.6% produced IMP.
Significance: In conclusion the data is in support of the spread of class B MBL’s into foods and is deserving of concern and immediate actions.
