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P2-04 Loop-mediated Isothermal Amplification Assays for Detecting Yersinia pseudotuberculosis in Milk Powders

Monday, July 27, 2015
Exhibit Hall (Oregon Convention Center)
Jinsong Feng , University of British Columbia , Vancouver , Canada
Xiaonan Lu , The University of British Columbia , Vancouver , Canada
Introduction: Yersinia pseudotuberculosis is a Gram-negative foodborne pathogen that causes several diseases, such as enteritis, septicemia, and reactive arthritis.

Purpose: Method for rapid detection of Y. peudotuberculosis in dairy products was developed.

Methods: Loop-mediated isothermal amplification (LAMP) assay targeting the 16S-23S rDNA internal transcribed spacer (ITS) region was developed to detect Y. pseudotuberculosis in milk powder.

Results: The DNA amplification could be completed in 1 h, and detected by produced white precipitate visible to naked eyes. The detection limit of LAMP assay was 100 fg/reaction for genomic DNA, and 100 CFU/100 g milk powder coupled with 12 h enrichment.
LAMP assay is 100 times more sensitive than conventional polymerase chain reaction method for detecting Y. pseudotuberculosis, and correctly identified 18 cases of Y. pseudotuberculosis contaminations from 236 commercial milk powder products.

Significance: The developed LAMP assay may facilitate rapid detection of Y. pseudotuberculosis contaminations in agricultural and food products.

Back to: Poster Session 2 – Laboratory and Detection Methods; Meat, Poultry and Eggs; Dairy and Beverages; Food Law and Regulation; General Microbiology; Modeling and Risk Assessment
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