P3-113 Bioactive Extracts of Berry Pomace and Probiotic Lactobacillus Synergistically Inhibit Pathogenic Salmonella

Tuesday, July 28, 2015
Hall B (Oregon Convention Center)
Serajus Salaheen, University of Maryland, College Park, MD
Mengfei Peng, University of Maryland, College Park, MD
Christine Mui, University of Maryland, College Park, MD
Cecilia Nguyen, University of Maryland, College Park, MD
Debabrata Biswas, University of Maryland, College Park, MD
Introduction: As the role of colonic microfloral composition in farm animal health are getting more attention, the improvement of gut flora with a combination of organic prebiotic-like substrates and probiotics is a critical area of interest. Berry pomaces are of interest to researchers because of their richness in several phytochemicals including proanthocyanidins, anthocyanins and other flavonoids. Partial degradation of phenolics by probiotics can increase bioavailability of these derivatives, hence combine effect of probiotic and its degradation activity of phenolic components will reduce pathogenic bacterial colonization in animal gut.

Purpose: To modulate the gut flora with synbiotic culture, we evaluated the role of bioactive extracts of berry pomace in growth of beneficial bacteria such as Lactobacillus plantarum and their inhibitory role against Salmonella Gallinarum and Salmonella Pullorum.

Methods: Growth performance of pathogens and probiotics were carried out in broth and fecal-medium. Co-culture of pathogen and probiotic was done in fecal medium in presence of berry pomace extract.

Results: We found that 2.0 mg GAE/ml of blackberry or blueberry pomace extract reduced the growth of Salmonella Gallinarum and Salmonella Pullorum by > 5 logs at 24 h in broth. In semisolid poultry fecal-medium, which represents simulated gut environment, 1.0 mg GAE/ml of both extracts reduced the growth of these pathogens by > 2 logs at 24, 48 and 72 h time points. The growth of probiotic L. plantarum was unaffected in the presence of berry pomace extracts in broth but growth stimulation was observed in fecal-medium at 72 h. Moreover, L. plantarum completely inhibited Salmonella Gallinarum and Salmonella Pullorum when co-cultured in fecal-medium in the presence of 1.0 mg GAE/ml of both pomace extracts. 

Significance: This study indicated that bioactive extracts from berry pomace are potential antimicrobials for organic producers which act synergistically with probiotic to reduce pathogen in simulated poultry gut condition.