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P2-162 Characterization of Equine-associated Escherichia coli Isolates in South Korea

Monday, July 27, 2015
Exhibit Hall (Oregon Convention Center)
Yeon Soo Chung , Seoul National University , Seoul , Korea, Republic of (South)
Jae Won Song , Seoul National University , Seoul , Korea, Republic of (South)
Dae Ho Kim , Seoul National University , Seoul , Korea, Republic of (South)
Young Kyung Park , Seoul National University , Seoul , Korea, Republic of (South)
Sook Shin , Seoul National University , Seoul , Korea, Republic of (South)
Sook Kyung Lim , Animal and Plant Quarantine Agency , Seoul , Korea, Republic of (South)
Yong Ho Park , Seoul National University , Seoul , Korea, Republic of (South)
Introduction: Escherichia coli (E. coli) is a part of the normal microflora of the gastrointestinal tract of animals and humans. According to the increased percentage of GDP, many people want to go out to enjoy their leisure time such as horseback-riding and horse meat. Imogen Johns et al reported that the isolation rate of ESBLs-producing E. coli from horse samples was over 60 percent.

Purpose: In this study, we examined the cross-transmission of antibiotic resistant (AR) E. coli between horses and their environments.

Methods: Isolated putative colonies from 3,078 swab samples were confirmed as E. coli by PCR method and antibiotic sensitivity of E. coli isolates was determined by a disk diffusion test with the 16 antibiotic disks according to the CLSI. In addition, PCR was performed to all isolates resistant to ampicillin, tetracycline, streptomycin and trimethoprim/sulfamethoxazole for screening the presence of the individual resistance genes. The genetic relatedness among the AR E. coli isolates was determined by PFGE.

Results: A total of 143 E. coli isolates (4.6%) were obtained from the swab samples and 30 isolates (21.0%) were resistant to at least one antibiotic. Most of AR E. coli isolates carried the corresponding resistance genes with minor exceptions. PFGE analysis with the 29 AR E. coli isolates showed that 2 clone sets identical in their molecular patterns were isolated from the 2 different sampling places.

Significance: The PFGE data indicated the possibility for cross-transmission of AR E. coli clones between horses and their environments. Therefore, we should examine the prevalence and antibiogram of equine-associated E. coli constantly.

Back to: Poster Session 2 – Laboratory and Detection Methods; Meat, Poultry and Eggs; Dairy and Beverages; Food Law and Regulation; General Microbiology; Modeling and Risk Assessment
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