Purpose: The objective of this study was to identify and characterize the Salmonella isolated in poultry carcasses directly transported from poultry slaughter house in Korea. In addition, we evaluated the performance of automated repetitive sequence-based PCR system (DiversiLabTM) for subtyping Salmonella isolated.
Methods: A total of 120 samples (60 of duck, 60 of chicken) were examined. Twenty-five grams of each sample were enriched in 225 ml of buffered peptone water and incubated for 24 h at 37°C. And then 0.1 ml of the enriched BPW was added to 9 ml of RV and incubated 24 h at 42°C followed by streaking onto XLD for Salmonella detection. After 24 h incubation at 37°C, presumptive colonies as Salmonella on XLD were confirmed by VITEKTM and with “O” antisera. In addition, an antibiotic resistance test was performed, and molecular subtypes of Salmonella isolates were ascertained using automated repetitive sequence-based PCR system (DiversiLabTM, BioMerieux, France).
Results: A total of 23 of 120 (19.2 %) Salmonella strains were isolated, and 11 of 23 (47.8 %) Salmonella strains were identified as serogroup D. On antibiotic resistance test, most of Salmonella were resistant to erythromycin and the other antibiotics tested. Automated repetitive sequence-based PCR system for molecular subtypes represented weak differentiation among the same serovar of Salmonella isolates, but good differentiation among different serovars.
Significance: Poultry products contaminated by Salmonella have the possibility to give the serious risk for human health. DiversiLabTM differentiated similar serogroups of Salmonella. The DiversiLabTM would facilitate timely public health recognition and response to foodborne disease outbreaks.