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P3-223 Pheno- and Genotypic Characteristics of Listeria monocytogenes Isolates from Various Foods

Tuesday, July 28, 2015
Hall B (Oregon Convention Center)
Jimyeong Ha , Sookmyung Women's University , Seoul , Korea, Republic of (South)
Sanghun Park , Seoul Metropolitan Government Research Institute of Public Health & Environment , Gyeonggi-do , Korea, Republic of (South)
Younghee Oh
Junghun Kim
Mokyoung Lee
Kweon Jung
Yohan Yoon , Sookmyung Women's University , Seoul , Korea, Republic of (South)
Introduction:  Listeria monocytogenes exists widely in the nature, and the pathogen can cross-contaminate fresh produce, Ready-to-Eat (RTE) meat and poultry, and fish. L. monocytogenes can cause 30% of mortality by intake of the contaminated foods.

Purpose:  The objective of this study was to analyze phenotypic and genotypic characteristics of L. monocytogenes isolated from different food groups.

Methods:  Chromosomal DNAs were extracted from 14 L. monocytogenes isolates from meat (7), RTE food (4), and fish samples (3). The presences of virulence genes (actA, inlA, inlB, plcB, and hlyA) were identified by PCR. Genetic correlations among the isolates were also evaluated by the PFGE (pulsed-field gel electrophoresis) patterns formed by Asc I.

Results: Of 14 isolates, most L. monocytogenes isolates possessed actA, inlA, inlB, plcB, and hlyA, and no differences in gene expression patterns of the virulence genes among sample groups (meat, RTE food, and fish) were observed. In general genetic correlations among the L. monocytogenes isolates mostly ranged 70% to 90%, and genetic categories among the sample groups were not detected.

Significance: This result indicates that L. monocytogenes strains isolated from various foods possess virulence genes, and genetic correlations among isolates were categorized by sample groups.

Back to: Poster Session 3 – Laboratory and Detection Methods, Sanitation; Antimicrobials; Produce; Epidemiology
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