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T11-09 Prevalence of Listeria monocytogenes in Slaughterhouses and Genetic Analysis among the Isolates Using Molecular Typing Methods

Tuesday, July 28, 2015: 10:45 AM
C124 (Oregon Convention Center)
Sejeong Kim , Sookmyung Women's University , Seoul , Korea, Republic of (South)
Jimyeong Ha , Sookmyung Women's University , Seoul , Korea, Republic of (South)
Soomin Lee , Sookmyung Women'S University , Seoul , Korea, Republic of (South)
Hye-Min Oh , Sookmyung Women's University , Seoul , Korea, Republic of (South)
Heui-Jin Kim
Yunsang Cho
Hee Soo Lee , Animal and Plant Quarantine Agency , Gimcheon , Korea, Republic of (South)
Jang Won Yoon , Kangwon National University , Chuncheon , Korea, Republic of (South)
Yohan Yoon , Sookmyung Women's University , Seoul , Korea, Republic of (South)
Introduction: Listeria monocytogenes has been recognized as an important foodborne pathogen. The pathogen has been isolated from fresh meat and poultry, and it can be transmitted to processed meats. Hence, the prevalence of L. monocytogenes in slaughterhouses needs to be evaluated.

Purpose: The objective of this study was to investigate the prevalence of L. monocytogenes in slaughterhouses and to analyze the genetic correlations among the isolates.

Methods: One hundred feces samples from cecum and 103 surface samples from 43 cattle and 60 pig carcasses were collected from 7 slaughterhouses, and 11 human isolates were obtained from hospitals. L. monocytogenes were identified by amplifying Listeria-specific genes (hly and prs) by PCR and eventually confirmed by 16s rRNA sequencing. The presence of virulence genes such as actA, inlA, inlB, plcB, and hlyAwere confirmed by PCR, and the serotypes were determined by multiplex-PCR and agglutination assay. Genetic correlations were also evaluated by the PFGE (pulsed-field gel electrophoresis) patterns formed by Asc I, and the PFGE patterns were compared among slaughter isolates and the slaughter isolate patterns were compared to the PFGE patterns from human isolates.

Results: Of 217 slaughterhouse samples, 12 samples (5.5 %; cattle: 4; pig: 8) were L. monocytogenes positive. All isolates had virulence-related genes such as actA, inlA, inlB, plcB and hlyA, and serotypes were generally1/2a, 1/2b, and 1/2c. Genetic correlations among slaughterhouse isolates mostly ranged 85% to 100%, and some of the isolates had genetic correlations with human isolates.

Significance: These results indicate that L. monocytogenes in slaughterhouse may be a potential source for human Listeriosis.

Back to: Technical Session 11 – Epidemiology
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