Purpose: Predictive models were generated by inoculating pacific white shrimps with V. cholerae, measuring viability rates at storage temperatures from 0 to 35°C, and fitting the data to a model to estimate growth parameters.
Methods: The models were evaluated with pacific white shrimps containing natural populations of V. cholerae. Viable V. cholerae was measured by pour plate method on thiosulfate-citrate-bile salts-sucrose agar for both inoculated and naturally contaminated shrimp samples. In parallel, total viable bacterial counts were measured by pour plate method on plate count agar.
Results: Growth/inactivation rates for V. cholerae were -0.008, -0.005, -0.002, 0.065, 0.125, and 0.252 log CFU/h at 0, 5, 10, 20, 30, and 35°C, respectively. The growth/inactivation rates for total viable bacteria were 0.007, 0.011, 0.024, 0.078, 0.130, and 0.322 log CFU/h at 0, 5, 10, 20, 30 and 35°C. Square root and Arrhenius models were generated for V. cholerae and total viable bacterial growth and inactivation kinetic data, respectively.
Significance: Evaluation studies showed that predictive growth for V. cholerae and total viable bacterial count were “fail-safe.” The models can be used by aquaculture establishments and regulators in implementing management strategies to minimize V. cholerae risk and enhancing product quality in supply chains.