S2 Metagenomic Analysis Pipelines: Really Learn about Your Food from Your NGS Data!

Sunday, July 26, 2015: 10:30 AM-12:00 PM
Oregon Ballroom 201 (Oregon Convention Center)
Primary Contact: Jennifer Patro
Organizers: Karen Jarvis , Andrea Ottesen and Jennifer Patro
Convenors: Karen Jarvis , Andrea Ottesen and Jennifer Patro
The audience will hear from bioinformatic experts from academia, food industry, and government that are working to develop and validate different sequencing approaches to characterize the microbial composition of fresh produce, probiotics and other foods and food environments.  Whole genome, 16S rRNA, and metagenomic sequencing are powerful tools to identify foodborne pathogens and improve understanding of how to prevent contamination. However, the large NGS data sets can present seemingly overwhelming bioinformatic challenges.  As the scientific community, particularly those involved in food safety embrace the many options for data generation and analysis – some degree of standardization needs to occur.  Methods for interpreting data will be presented and the road to validation for certain approaches will be discussed. This symposium will highlight some of the analysis platforms currently used to identify pathogens and characterize ecologies that play a role in contamination.

Presentations

11:00 AM
Metagenomic Applications for Characterizing the Food Microbiome
David Chambliss, IBM Almaden Research Center; Stefan Edlund, IBM Almaden Research Center
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