Purpose: This study evaluatee the inhibitory effects of D-tryptophan on Escherichia coli O157:H7 and Salmonella enterica under various mixed NaCl and D-tryptophan stress conditions and, subsequently, test its antibacterial efficacy against E. coli O157:H7 in freshly shucked oysters.
Methods: We examined the antibacterial effect of D-tryptophan on E. coli O157:H7 and S. enterica in liquid media with various NaCl concentrations. A model identifying the boundary between growth/no growth of the bacteria as a function of NaCl and D-tryptophan concentration was developed by logistic regression with R statistical software. In addition, freshly shucked oysters were artificially contaminated with E. coli O157:H7 in artificial seawater (3.5% NaCl) with or without 40 mM D-tryptophan. Bacterial survival was enumerated using plate counts.
Results: Growth/no growth boundary models were successfully developed for both pathogens. Salmonella enterica and E. coli O157:H7 growth was affected at NaCl concentrations >3% and 4%, respectively, in the presence of 20 mM D-tryptophan; but higher concentrations of both substrates were needed for efficient growth inhibition. In addition, adding D-tryptophan significantly inhibited E. coli O157:H7 growth in freshly shucked oysters.
Significance: D-tryptophan may act as a novel, natural food preservative to control foodborne bacterial growth, protect low aw food quality, and extend the shelf-life of processed seafood.