P1-220 A Survey of Antimicrobial Resistance among Dairy Cattle in Kosovo

Monday, July 10, 2017
Exhibit Hall (Tampa Convention Center)
Sulaiman Aljasir , University of Wyoming , Laramie , WY
Jeffrey Chandler , U.S. Department of Agriculture-APHIS-WS-NWRC , Fort Collins , CO
Afrim Hamidi , University of Pristina , Pristina , Kosovo
Driton Sylejmani , University of Pristina , Pristina , Kosovo
Baolin Wang , University of Wyoming , Laramie , WY
Katherine Schwam , University of Wyoming , Laramie , WY
Bledar Bisha , University of Wyoming , Laramie , WY
Introduction: The overuse and abuse of antimicrobials during food production is a threat to public health. Antibiotics are routinely used in dairy cattle to treat mastitis, respiratory infections and other conditions and may ultimately play a role in the emergence and dissemination of antimicrobial resistance in the food supply.

Purpose: The purpose of this study was to conduct a survey of antimicrobial resistance in dairy cattle in Kosovo through isolation and characterization of the indicator microorganisms Escherichia coli and Enterococcus spp.

Methods: A cross-sectional study was conducted in the summer of 2014 on dairy farms representing the majority of dairy production regions of Kosovo. Fresh fecal samples were collected from 10 cattle in each of the 52 farms included in the study. Individual samples were mixed to form composite fecal samples (500 g) for each farm (n = 52). Isolation was performed on McConkey agar (E. coli) and Enterococcosel agar (Enterococcus spp.) or media supplemented with ciprofloxacin, erythromycin, or cefoxitin. Isolate confirmation was carried out by MALDI Biotyping. Antimicrobial susceptibilities were determined via the disk diffusion method according to CLSI guidelines.

Results: The highest percentage of resistant E. coli isolates (n = 165) were to ampicillin (29.7%), followed by cefazolin (26%) and tetracycline (19.4%). Notably, resistance was also high for piperacillin (17.6%), nalidixic acid (17%) and ciprofloxacin (13.3%). Among enterococci (n = 177), E. faecalis isolates were primarily resistant to erythromycin (36.5%), rifampin (33.8%), tetracycline (28.4%) and ciprofloxacin (27%). Similar trends were observed for E. faecium, with high levels of resistance to rifampin (52%), tetracycline (38%), ciprofloxacin (36%), doxycycline (28%) and erythromycin (20%).

Significance: This is the first survey of antimicrobial resistance in dairy operations of Kosovo, providing a baseline of antimicrobial resistance within this important niche of food production.