Thursday, May 12, 2016: 2:30 PM
Mc3 (Megaron Athens International Conference Center)
Manuel Jimmy Saint-Cyr, ONIRIS/INRA, UMR 1014 Secalim, LUNAM Université, Nantes, France
Muriel Guyard-Nicodème, French Agency for Food, Environmental and Occupational Health and Safety, Ploufragan, France
Bernard Taminiau, Food Science Department, FARAH, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
Michel Amelot, ANSES, Laboratory of Ploufragan-Plouzané, European University of Brittany, Ploufragan, France
Georges Daube, Food Science Department, FARAH, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
Xavier Dousset, ONIRIS/INRA, UMR 1014 Secalim, LUNAM Université, Nantes, France
Nabila Haddad, ONIRIS/INRA, UMR 1014 Secalim, LUNAM Université, Nantes, France
Campylobacteriosis is the most frequently reported zoonotic disease in humans in the European Union, and poultry meat the main source of human infection. Several strategies to reduce the prevalence and colonization of
Campylobacter in broiler chickens are being developed in the primary production, including the use of probiotics. Recent advances will be presented with a focus on
Lactobacillus salivarius SMXD51. SMXD51, a potential probiotic strain shows an
in vitro and
in vivo anti-
Campylobacter activity. But, the delivery route and the administration form are inconsistent for an industrial application. Freeze-dried
SMXD51 were produced from a laboratory (LFD-SMXD51) and an industrial (IFD-SMXD51) scale.
The aim of the study was to assess i) the competitive exclusion of Campylobacter jejuni in broiler chickens by freeze-dried SMXD51, and ii) the influence of the freeze-drying processes on physiological parameters and probiotic abilities of SMXD51 through in vitroassays.
Administration of freeze-dried SMXD51 showed variable impact on Campylobacter colonization. Per os treatment with LFD-SMXD51 leaded to a significant reduction of cecal C. jejuni loads by 1.5 log (P < 0.001) in broilers of 35 days of age while no effect was observed in another trial. IFD-SMXD51 treatments (per os or in feed) showed no effect on Campylobacter colonization. In vitrocomparison of the freeze-dried forms with the native strain showed differences in membrane cell properties and probiotics abilities.
Literature highlights interesting but variable results on reduction of Campylobacter levels in broiler chickens. Our results suggest that freeze-drying processes and differences of avian gut microbiota are two factors that could influence the in vivo anti-Campylobacter activity of SMXD51.
