Purpose: Here we present the use of the Maxwell® RSC PureFood GMO and Authentication kit to purify amplifiable DNA for PCR-based genetically modified organism (GMO) and food authentication testing.
Methods: DNA, purified from beef, pork, canola, corn meal, soy, and cereal using Maxwell® RSC PureFood GMO and Authentication, DNeasy® mericon® Food, and r-biopharm SureFood® kits, was analyzed for yield, purity, and amplifiability using GoTaq® qPCR. GMO maize was spiked into GMO-free maize at 10, 1, 0.1, 0.01, and 0.001% (w/w). DNA was, then, purified using the Maxwell® RSC PureFood GMO and Authentication kit and the DNeasy® mericon® kit. DNA eluates were amplified, using the TaqMan® GMO Maize 35S detection kit, to identify the percent GMO in the samples. For authentication testing, beef was spiked with pork at 10, 1, 0.1, 0.01 and 0.001% (w/w). DNA from samples was purified, using the Maxwell® RSC PureFood GMO and Authentication Kit and the DNeasy® mericon® Food kit. DNA eluates were amplified using the RapidFinder™ Pork ID kit to identify swine DNA.
Results: All three kits provided amplifiable DNA. For GMO testing, the 35S event was detected down to 0.01% in the PureFood samples and down to 0.1% in the DNeasy® mericon® samples. For authentication testing, DNA was amplified with the RapidFinder™ Pork ID kit from all samples, except the 100% beef sample.
Significance: These studies, together, demsontsrate the utility of the Maxwell® RSC for automated purification of food DNA for amplification-based GMO and authentication testing.