P2-105 Development of a Selective Enrichment Broth Supplemented with Bacteriological Charcoal and a High Concentration of Polymyxin B for the Detection of Campylobacter jejuni and Campylobacter coli in Chicken Carcass Rinse

Tuesday, July 30, 2013
Exhibit Hall (Charlotte Convention Center)
Jung-Whan Chon, Konkuk University, Seoul, South Korea
Jin-Hyeok Yim, Konkuk University, Seoul, South Korea
Jun-Ho Park, Konkuk University, Seoul, South Korea
Hong-Seok Kim, Konkuk University, Seoul, South Korea
Dong-Hyeon Kim, Konkuk University, Seoul, South Korea
Jong-Soo Lim, Konkuk University, Seoul, South Korea
Kwang-Young Song, Konkuk University, Seoul, South Korea
Kun-Ho Seo, Konkuk University, Seoul, South Korea
Introduction: A new Campylobacter-selective enrichment broth supplemented with bacteriological charcoal and a high concentration of polymyxin B was developed (charcoal-cefoperazone-polymyxin B-deoxycholate broth; CCPD broth).

Purpose: The ability of CCPD broth to be used for the detection of Campylobacter jejuni and Campylobacter coli in chicken carcass rinse was compared to that of modified Bolton (mBolton) broth.

Methods: A total of 80 whole chickens purchased from retailers were rinsed with 400 ml buffered peptone water. The rinse samples were enriched with 2 × blood-free mBolton enrichment broth and 2 × CCPD broth at 42°C for 48 h and then streaked onto modified charcoal-cefoperazone-deoxycholate agar (mCCDA). Suspected colonies were confirmed by colony PCR.

Results: The Campylobacter isolation rate was significantly (P < 0.05) higher in the CCPD broth than in the mBolton broth (CCPD broth, 61 out of 80; mBolton broth, 34 out of 80). Moreover, the selectivity of CCPD broth agar was also superior (P < 0.05) to that of the mBolton broth when comparing the number of contaminated mCCDA plates (CCPD broth, 16 out of 80; mBolton broth, 58 out of 80) and the growth index of competing flora (CCPD broth, 1.4; mBolton broth, 2.9).

Significance: Significant elimination of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in CCPD broth was also observed.