Purpose: In this study, two novel selective medium were compared with MYPA for the isolation and enumeration of B. cereus in naturally contaminated fermentation sauce samples.
Methods: A total of 46 fermentation sauce samples were purchased from a local retail market. Each sauce sample (25 g) was suspended in 225 ml of Butterfield’s phosphate-buffered water and homogenized for 2 min. Subsequently, 1 ml of homogenate was serially diluted (10-fold) in PBS followed by the inoculation of 0.1 ml of each dilution onto three plating media in duplicate. MYPA and mMYPA were incubated at 30°C and BYPTA plates at 37°C for 24 h.
Results: No statistical difference was observed in the quantitative enumeration (P > 0.05) between three selective media, whereas BYPTA and mMYPA exhibited higher selectivity than MYPA excluding the background flora in fermentation food samples.
Significance: Two novel selective medium could be a useful enumeration tool to assess the level of B. cereus in various fermentation sauce samples.