Purpose: The aim of this study was to develop a more sensitive, specific and rapid technique of loop-mediated isothermal amplification (LAMP) for detecting of Arcobacter species.
Methods: The LAMP reaction was optimized under different conditions. Specificity and sensitivity of LAMP were compared with multiplex polymerase chain reaction. Bacterial isolation, multiplex PCR, and LAMP were performed in chicken samples collected from retailed markets.
Results: The optimization reaction condition for LAMP was achieved at 61°C for 50 min and ladder-like DNA products were produced with reference strains and field isolates of Arcobacter species. Because multiplex PCR showed the cross-reactivity with Campylobacter species, the LAMP assay was specific rather than multiplex PCR which was used in previous studies. The detection limit of LAMP was 2-20 CFU/reaction in vitro and 200 CFU/reaction in chicken samples. The sensitivity of LAMP assay showed a tenfold to thousand-fold times more than that of multiplex PCR assay.
Significance: The LAMP assay developed in this research is rapid and reliable detection technique for Arcobacter species which can cause food poisoning in human.