Purpose: This study was performed to demonstrate comparative enumeration of yeast and molds using a new dehydrated film medium and reference methodology as described in FDA/BAM and ISO21527:2008 (parts 1 and 2).
Methods: The method comparison was conducted using a variety of naturally contaminated food matrices (n = 75) with the new dehydrated film test method and a reference method (FDA/BAM and ISO21527:2008, parts A and B). Each food sample was serially diluted in 0.1% peptone water. The new dehydrated film medium was hydrated with 1ml of the sample and 100 micro-liters were spread plated on DRBC or DG-18 media in replicate.
Results: Results were statistically comparable between the new dehydrated film method at 48-60 h to DRBC and DG-18 at 5 days with no significant differences as indicated by the P-values (> 0.05) and regression analysis.
Significance: Rapid quantitative detection of yeasts and molds using the new dehydrated film method was evaluated using a large number (n = 75) of naturally contaminated foods. The new method was found to be reliable and have improved interpretation thus providing end users with actionable results in a shorter time frame.