Purpose: The purpose of this study was to determine the influence of ripeness, bruising and storage temperature on the survival of foodborne pathogens on the surface of Bartlett pears.
Methods: Bartlett pears at two stages of ripeness (8 kgf (hard) and 3 kgf (soft)) were bruised using a standard method. Both unblemished and bruised surfaces (3 cm2) were inoculated with five-strain cocktails of rifampicin-resistant Salmonella or Escherichia coli O157:H7 at a level of 4 log CFU/3 cm2. Pears were stored at 1 and 20°C and 90% RH for up to 48 h. The inoculated area was excised to recover surviving populations, samples were diluted in 0.1% peptone and plated onto specific and non-specific media containing rifampicin or, when appropriate, enriched using standard methods.
Results: In all cases, population densities of Salmonella or E. coli O157:H7 decreased by 1 to 2 log CFU within the first 2 h and were near or below the limit of detection (1 CFU/3 cm2) by 24 h. The percent positive samples (of 12) at 48 h ranged from 0 to 100%; greater survival was associated with harder fruit. For Salmonella, the number of positive samples was identical for unblemished and bruised fruit except for soft pears stored at 20°C; at that temperature Salmonella was isolated more frequently from soft unblemished fruit. For E. coliO157:H7 greater survival was consistently noted for bruised pears (both hard and soft).
Significance: Shipping riper pears should not increase food safety risks associated with Salmonella or E. coli O157:H7.