P3-142 Baseline of Salmonella Prevalence in Retail Beef and Produce from Honduras and Mexico

Wednesday, July 31, 2013
Exhibit Hall (Charlotte Convention Center)
Martha Maradiaga, Texas Tech University, Lubbock, TX
Markus Miller, Texas Tech University, Lubbock, TX
Leslie Thompson, Texas Tech University, Lubbock, TX
Alejandro Echeverry, Texas Tech University, Lubbock, TX
Lyda Garcia, Texas Tech University, Lubbock, TX
Mindy M. Brashears, Texas Tech University, Lubbock, TX
Sara Gragg, Texas Tech University, Lubbock, TX
Alexandra Calle, Texas Tech University, Lubbock, TX
Ansen Pond, Texas Tech University, Lubbock, TX
Shanna Ward, Texas Tech University, Lubbock, TX
Introduction: Salmonella continues to cause a considerable number of foodborne illnesses worldwide, with recent outbreaks reported. The sources of these latest outbreaks include contaminated meat and produce.  A comprehensive baseline of the Salmonella contamination in Mexico and Honduras has not been previously reported.

Purpose: To establish a baseline of Salmonella prevalence in Mexico and Honduras by sampling produce and beef from retail markets and abattoirs.

Methods: Retail produce samples (cantaloupes, cilantro, cucumbers, leafy greens, peppers, and tomatoes) were purchased in Mexico (n = 576) and Honduras (n = 479), retail whole muscle beef (n = 555) samples were purchased in Honduras and both hide and beef carcass (n = 141) samples were collected from 2 Honduran abattoirs. Beef samples were obtained using a sponge hydrated with buffered peptone water (BPW) and a BPW rinsate of produce samples was collected and transported back to the U.S.  Salmonella was detected using standard protocols for the Dupont Qualicon BAX®system and positive samples were isolated using traditional cultural methods and confirmed via agglutination and serotyping.

Results: Overall, the prevalence of Salmonella-positive samples in Honduras (n=555) retail beef was 1.0% (95% CI = 0.8, 1.3), whereas 7.8% (n = 141) of beef carcass swabs were positive in both Honduran beef plants. The overall Salmonella prevalence for produce samples was 2.1% (95% CI = 1.2, 3.6) and 1.6% (95% CI = 1.2, 3.8) for Honduras (n = 573) and Mexico (n = 514), respectively. The most common serotypes identified in Honduras were Salmonella Typhimurium followed by Derby, while Meleagridis, Typhimurium, Kentucky, and Newport were commonly isolated from Mexico.

Significance: Although the prevalence of Salmonella was low in this study, Salmonella still continues to be a challenge for the food industry worldwide. Developing a Salmonella baseline for Latin America provides understanding of the worldwide burden, thus providing insight into foodborne Salmonella control.