Start | Browse by Day | Author Index | Personal Scheduler

P3-79 Antibiotic Susceptibility of Listeria Species Isolated from Conventional and Pasture Flock Raised Poultry and Their Environments

Wednesday, August 6, 2014
Exhibit Hall D (Indiana Convention Center)
Hao Shi, University of Arkansas, Fayetteville, AR
Si Hong Park, University of Arkansas, Fayetteville, AR
Nathan Jarvis, University of Arkansas, Fayetteville, AR
Janet Donaldson, Mississippi State University, Starkville, MS
Philip Crandall, University of Arkansas, Fayetteville, AR
Steven Ricke, University of Arkansas, Fayetteville, AR
Introduction: Listeria monocytogenes has been recognized as a foodborne pathogen of public health concern in the food industry since it causes severe diseases in immuno-compromised persons and pregnant women. Due to the increasing emergence of drug resistant bacterial species, identification and investigation of antibiotic resistant Listeria species isolated from foods and environments should be conducted to monitor the emergence and spread of antibiotic resistance in food environments.

Purpose: The aim of this study was to evaluate the antibiotic susceptibility of Listeria species including L. monocytogenes isolated from conventional versus antibiotic-free pastured flock poultry and environments.

Methods: A total of 29 Listeria strains from four species (L. monocytogenes, L. innocua, L. welshimeri and L. seeligeri) isolated from conventional and pasture flock raised poultry and their environments were evaluated using 7 antibiotics (ampicillin(10 µg), ampicillin-sulbactam (10/10 µg), ciprofloxacin (5 µg), gentamicin (10 µg), kanamycin (30 µg), imipenem (10 µg) and tetracycline (30 µg)). These antibiotics are representatives of report group based on CLSI guideline.

Results: Most Listeria strains were susceptible to the antibiotics tested but 5 strains were resistant to at least one antibiotic. Two pastured poultry L. innocua strains isolated from soil showed resistance against ampicillin and ampicillin-sulbactam, and tetracycline, respectively. One pastured poultry L. welshimeri strain isolated from a chicken carcass rinsate exhibited Tetracycline resistance. Furthermore, one pastured poultry L. monocytogenes from grass showed ampicillin and one L. monocytogenes from environment exhibited ampicillin-sulbactam resistance, respectively.  

Significance: Investigation of antibiotic response profiles in Listeria species in conventional poultry and pasture flock environments are important to monitor spreading of resistant strains to establish prevalence and origins of antibiotic resistance in food system environments.

Back to: Poster Session - Antimicrobials, Meat and Poultry, Microbial Food Spoilage, Seafood, Sanitation, Non-microbial Food Safety, Risk Assessment
<< Previous Abstract | Next Abstract >>