Purpose: This study tested the hypothesis that GAC filters could serve as a reservoir for Cronobacter by trapping low levels of nutrient upon which the organism could grow.
Methods: A simulated water filter system was installed by filling glass tubes with sterile GAC, followed by sterile water with a dilute carbon source (peptone) flowing through the column at a rate of 1 liter/day. Carbon columns were inoculated with 104 CFU of Cronobacter on the surface, and the effluent monitored for Cronobacter levels. During a second phase, commercial faucet filters will be distributed to households for 4-month use. Used filters were backwashed with sterile peptone water, and analyzed for Cronobacter, total aerobic plate count, coliform bacteria and Enterobacteriaceae.
Results: Cronobacter colonized the simulated GAC and grew when provided minimal levels of nutrients, shedding up to 107 CFU/ml in the effluent water after 2 days. Most Cronobacter colonized on the upper part of the column. Backwashed used filters used in home settings yielded presumptive Cronobacter spp., Escherichia coli, Pseudomonas and other waterborne bacteria.
Significance: Cronobacter and other bacteria are able to colonize on granular activated carbon filter, penetrate the treatment barrier and release into water effluent, introducing a safety risk of waterborne pathogens, and potentially contaminating the kitchen environment. These results suggest that the use of these filters should be considered a potential source of Cronobacter and other foodborne pathogens under certain circumstances.