Purpose: The objective of this research is to evaluate whether this recommendation is sufficient to inactivate two HNV surrogates, Murine Norovirus (MNV-1) and Tulane virus (TV) in oyster meats.
Methods: Oyster homogenates were inoculated with ~5 log PFU/ml MNV-1 and ~3 log PFU/ml TV, respectively. The inoculated samples were treated in a circulating water bath maintained at 49, 54, 58, 63, 67°C for different durations. First-order model was used for calculating the D values of MNV-1 and TV at different temperatures. The oyster homogenates were also treated in a boiling water bath for 1, 2 and 3 min.
Results: D-values were 28.17 to 0.88 min for MNV-1, while they ranged from 18.18 to 1.56 min for TV in oyster homogenates treated at 49 to 67 °C. The kinetics demonstrated that TV was much more heat sensitive (P<0.05) compared with MNV-1, which might make it a less comparable virus surrogate as HNV in cooked oyster meats. One minute of boiling water had 0.73 and 1.22 log reduction for MNV-1 and TV, respectively, while 2 minutes of the treatment produced 3.64 log reduction for MNV-1 and below detect limitation (1 log PFU/ml) for TV. After 3 minutes of boiling, the titer of MNV-1 also dropped <1 log PFU/ml.
Significance: MNV-1 is more heat resistant than TV in oyster homogenates. Boiling the oyster meats for 3 minutes may be effective to inactivate HNV to below 1 log PFU/ml.