P2-169 Microbiological Quality of Street-Vended Juices in Jeli District, Malaysia

Tuesday, August 2, 2016
America's Center - St. Louis
Soo Yee Chong, Universiti Malaysia Kelantan, Jeli, Malaysia
Pasupuleti Visweswara Rao, Universiti Malaysia Kelantan, Jeli, Malaysia
Carol Wallace, University of Central Lancashire, Preston, United Kingdom
Jan Mei Soon, University of Central Lancashire, Preston, United Kingdom
Introduction: Street-vended beverages are widely accepted by consumers in Malaysia due to their taste, reasonable prices, and all-time availability. Freshly prepared juices however have no process or steps to minimize foodborne pathogens if they are contaminated.

Purpose: It is the purpose of this study to determine the microbiological properties of street-vended sugarcane juice and coconut water and associated preparation surfaces sold by three selected street vendors in Jeli, Malaysia.

Methods: A total of 18 beverages and 15 swab samples with 47 direct film samples were collected from 3 stalls in Jeli district, Kelantan, Malaysia. Coliforms and Escherichia coli were chosen as the safety indicator and microbiological content of beverages; whereas, Staphylococcus load represented the hygienic practices of vendors. Selective agar was used for conventional enumeration of microbial load while Sanita-kun dehydrated medium was used for direct stamping and enumeration of Escherichia coli and Staphylococcus aureus.

Results: Sugarcane juice recorded the highest bacterial contamination at 2.63±0.01 log CFU/ml) and coconut water at 2.56±0.01 log CFU/ml from street vendor B. The sugarcane extractor from Stall B also recorded the highest total viable counts (4.13±0.01 log CFU/ml). The results indicated that 26.67% of the preparation surfaces samples have presumptive Escherichia coli detected whereas stall B handler’s apron had the highest Staphylococcus count on both Baird-Parker Agar and Sanita-kun dehydrated medium at 3.08±0.01 log CFU/ml and 3.11±0.01 log CFU/ml, respectively.

Significance: Street-vendors should practice Good Hygiene Practices (GHPs) in preparation and handling of beverages for consumption. Regular hygienic assessment and monitoring of the quality of street-vended beverages are required to ensure food hygiene and avoid foodborne illnesses.