P1-116 Evaluation of Molecular Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium Assay Performance in Poultry Meat Samples

Monday, August 1, 2016
America's Center - St. Louis
Jani Holopainen, Thermo Fisher Scientific, Vantaa, Finland
James Stringer, Thermo Fisher Scientific, Basingstoke, United Kingdom
Katharine Evans, Thermo Fisher Scientific, Basingstoke, United Kingdom
Mikko Kauppinen, Thermo Fisher Scientific, Vantaa, Finland
Introduction: Thermo Scientific TaqMan Salmonella Triplex Assay with lysis is a PCR based test for the simultaneous detection and differentiation of Salmonella species, Salmonella Enteritidis and Salmonella Typhimurium in food and environmental samples. The TaqMan Salmonella Triplex Assay workflow includes a simple 15-minute sample lysis step followed by a 40-minute PCR run on Applied Biosystems 7500 Fast Real-Time PCR instrument.

Purpose: The purpose of the study was to verify performance of the Salmonella Triplex Assay with a range of different poultry samples and compare the results to those obtained with the DuPont BAX System Real-Time Salmonella Assay or bioMérieux VIDAS-UP Salmonella Assay at two major poultry meat manufacturers’ laboratories in Brazil.

Methods: A total of 152 naturally contaminated poultry samples representing 13 different matrix types were tested at Poultry Meat Manufacturer 1 (PMM 1). Additionally, 12 poultry samples representing 2 matrix types were inoculated with Salmonella Enteritidis or Salmonella Typhimurium at Poultry Meat Manufacturer 2 (PMM 2). Three samples were inoculated with a competing background of either Escherichia coli or Enterobacter aerogenes. All samples analyzed with the Salmonella Triplex kit were enriched in Buffered Peptone Water at 37°C for 18 hours, lysed, tested and analyzed according to the protocol described for the TaqMan Salmonella Triplex Assay with lysis. The BAX or VIDAS-UP Salmonellaanalysis was performed according to the manufacturers’ instructions.

Results: The Salmonella Triplex Assay method proved to be an accurate method. One hundred forty-nine of the 152 samples tested (98.0%) yielded comparable results with the BAX and VIDAS-UP methods at PMM 1 laboratory and 100%  (12/12) of samples gave the correct results in the analysis conducted at PMM 2.

Significance: The study demonstrated that the TaqMan Salmonella Triplex Assay method offers a rapid, easy-to-use and reliable workflow for the detection of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium in poultry samples.