Navigation

P2-38 Improvement of Polymyxin-Egg Yolk-Mannitol Bromothymol Blue Agar for the Enumeration and Isolation of Bacillus cereus in Various Foods

Tuesday, August 2, 2016
America's Center - St. Louis
Il-Byeong Kang, Konkuk University, Seoul, Korea, The Republic of
Jung-Whan Chon, U.S. Food and Drug Adiministration, Jefferson, AR
Dong-Hyeon Kim, Konkuk University, Seoul, Korea, The Republic of
Hong-Seok Kim, Konkuk University, Seoul, Korea, The Republic of
Kun-Ho Seo, Konkuk University, Seoul, Korea, The Republic of
Introduction: Polymyxin-egg yolk-mannitol-bromothymol blue (PEMBA) has been recommended for the enumeration and isolation of B. cereus by food authorities.

Purpose: We developed modified PEMBA by supplementing trimethoprim to PEMBA to improve the selectivity for Bacillus cereus in various foods.

Methods: Field samples including red pepper powder (n=10), fermented soybean paste (n=10), Saengsik (n=10), Sunsik (n=10), and fruit juice (n=10) were used to evaluate the performance of the novel media compared to PEMBA.

Results: In red pepper powder and soybean paste, the number of B. cereus in mPEMBA were significantly higher than that in PEMBA indicating better recoverability (P < 0.05). In addition, mPEMBA provided better visual differentiation of B. cereus colony than PEMBA, which is attributable to reduced number of competing flora.

Significance: In conclusion, the addition of trimethoprim to PEMBA could generate a synergistic effect to improve selectivity, suggesting mPEMBA could be a great alternative to PEMBA in isolating and enumerating B. cereus in various foods.

Back to: Poster Session 2 – Low-water Activity, Laboratory and Detection Methods, Epidemiology, Produce, Pre-harvest, Dairy and Beverages, Food Defense, General Microbiology
<< Previous Abstract | Next Abstract >>