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P1-194 Inhibitory Effect of Herbal Extracts against Hepatitis A Virus

Monday, August 1, 2016
America's Center - St. Louis
Dong Joo Seo, Chung-Ang University, Ansung, Korea, The Republic of
Su Been Jeon, Chung-Ang University, Ansung, Korea, The Republic of
Hyejin Oh, Chung-Ang University, Ansung, Korea, The Republic of
Yeonmoon Jeong, Chung-Ang University, Ansung, Korea, The Republic of
Hyunkyung Park, Chung-Ang University, Ansung, Korea, The Republic of
Suntak Jeong, Chung-Ang University, Ansung, Korea, The Republic of
Changsun Choi, Chung-Ang University, Ansung, Korea, The Republic of
Introduction: Hepatitis A virus (HAV) is positive-sense and single-stranded RNA virus of the Picornaviridae family and cause acute liver infection and gastrointestinal infections through the fecal-oral route. Although antimicrobial activity of herbal extracts was studied against bacterial pathogens, it is rare to investigate anti-HAV property of food components.

Purpose: The aim of this study was to examine the inhibitory effect of 16 edible herbal extracts against HAV.

Methods: S. flavescens, C. officinalis, V. vinifera, P. lactiflora, A. thumbergii, E. ulmoides, E. sessiliflorus, P. multiflorus, C. sativum, G. biloba, A. fistulosum, A. annua, A. sativum, T. japonica, A. pilosa, A. japonica were used to prepare herbal extract in this study. Antiviral activity, cytotoxicity, and antioxidant activity was measured on FRhK-4 Cells for HAV at 1, 10, and 50 µg/ml concentration of plant extracts. The pre-, co-, and post-treatment of plant extracts was investigated on HAV-infected cells.  

Results: Viral inhibition was observed by co-treatment than pre- and post-treatment. The effect of A. japonica of 50 µg/ml showed the significant reduction of 3.36 ± 0.17 log PFU/ml of HAV without cytotoxicity. The titer of HAV significantly reduced to 1.84 ± 0.24, 1.77 ± 0.35, and 1.74 ± 0.10 log PFU/ml on FRhK-4 Cells co-treated with 50 µg/ml of A. pilosa, T. japonica, and A. sativum, respectively. Also, 50 µg/ml of E. sessiliflorus, P. multiflorus, C. sativum, G. biloba, A. fistulosum, and A. annua showed the reduction about 1 log PFU/mL against HAV.

Significance: The herbal extracts of A. japonica, A. pilosa, T. japonica, and A. sativum could have potential to control HAV without cytotoxicity.

Back to: Poster Session 1 – Produce, Meat, Poultry and Eggs, Non-microbial Food Safety; Laboratory and Detection Methods; Communication Outreach and Education; Seafood; Antimicrobials; Food Toxicology
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