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P3-86 Simultaneous Detection and Prevalence of Allergens in Anisakis Species Isolated from Sea Fish

Wednesday, August 3, 2016
America's Center - St. Louis
Woo Joo Lee, Chung-Ang University, Ansung, Korea, The Republic of
Dong Joo Seo, Chung-Ang University, Ansung, Korea, The Republic of
Hyejin Oh, Chung-Ang University, Ansung, Korea, The Republic of
Su Been Jeon, Chung-Ang University, Ansung, Korea, The Republic of
Day Jung, Chung-Ang University, Ansung, Korea, The Republic of
Changsun Choi, Chung-Ang University, Ansung, Korea, The Republic of
Introduction: Anisakis simplex was first reported in the Netherlands in 1960 and over 20,000 cases of anisakiasis have been reported worldwide. In Korea, 107 cases of human anisakiasis to cause acute abdominal pain were reported. Since the allergenic potential of A. simplex was first addressed in Japanese urticaria patients who consumed mackerel, urticaria was known as a common allergic symptom in Anisakis allergy cases. While most of the previous studies have focused on the identification and characterization of individual Ani s allergens in A. simplex larvae, the prevalence and profiles of Ani s allergens have not yet been examined so far in anisakid larvae.

Purpose: This study aimed to develop a multiplex reverse transcription polymerase chain reaction (RT-PCR) for the detection of Anisakis allergens and to investigate the relationship of allergen profiles and anisakid larvae isolated from Scomber japonicus, Trichiurus lepturus, and Conger myriaster in Korea.

Methods: The visceral organs of 49 mackerel (Scomber japonicus), 45 large head hairtail (Trichiurus lepturus), and 46 whitespotted conger (Conger myriaster) were collected as samples from seafood markets and retailers. The Anisakis species was determined by Anisakis pegreffii-specific PCR and polymerase chain reaction-restriction fragment length polymorphism analysis. The prevalence and profile of 5 Ani s allergens were examined by multiplex RT-PCR.

Results: Among 140 larvae, A. pegreffii and A. typica accounted for 97.1% and 2.9%, respectively. The prevalence of each allergen in A. pegreffii was 41.2% for Ani s 1, 72.1% for Ani s 2, 69.9% for Ani s 3, 86.7% for Ani s 4, 93.4% for Ani s 5. Most A. pegreffii larvae had multiple allergen profiles, and 80.7% of A. pegreffii carried both Ani s 4 and Ani s 5 that are heat-resistant allergens. Especially, 52~65% of A. pegreffii isolated from S. japonicus and C. myriaster carried all 5 Ani s allergens.

Significance: This study clearly demonstrated the prevalence and profiles of Ani s allergens that have allergenic potential in A. pegreffii and A. typica larvae.

Back to: Poster Session 3 – Antimicrobials, Laboratory and Detection Methods, Microbial Food Spoilage, Modeling and Risk Assessment, Retail and Food Service Safety and Sanitation
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