Purpose: For microbiological reduction of OTA in fermented soybean products, fungi that have OTA-biodegradation activity were isolated from meju, a traditional starter for Korean soybean fermented products. The OTA-biodegradation activity of 7 fungi and their crude enzymes was investigated.
Methods: One hundred thirty fungal species were isolated from meju and their OTA-biodegradation activity was evaluated. Biodegradation of OTA was analyzed by thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC). Seven fungal isolates that show OTA-biodegradation activity were identified by sequencing β-tubulin gene and the Internal Transcribed Spacer (ITS) region of rDNA.
Results: Seven Aspergilli were selected as OTA-biodegradable abilities and identified as A. tubingensis M013, M024, M036, M038, M069 and M074. They degraded more than 90% of OTA in 14 days in Soytone-Czapek broth and converted OTA to a much less toxic metabolite, ochratoxin α. Their crude enzymes degraded more than 85% and 75% of OTA at pH 5 and pH 7, respectively, for 24 h.
Significance: Our data suggests that A. tubingensis M013, M024, M036, M038, M069 and M074 isolated from meju samples and their OTA-biodegradable enzymes have potential for practical applications to reduce OTA levels in soybean fermented food and feed processing.