Purpose: In this study, we sought to investigate the safety of free-range chickens that are available to purchase by consumers in the U.S.
Methods: Chicken hearts were sampled from the local markets in Maryland and from farms in Maryland and Amish community in Chicago. Each seropositive (modified agglutination test (MAT) positive) heart was bioassayed using Swiss Webster (SW) mice and examined for T. gondii infection. In addition, thirteen cryopreserved isolations from previous studies were revived and ten PCR-RFLP markers were used to genotype those isolates.
Results: One hundred fifty from a total of 997 samples (15.0%) were found seropositive for T. gondii. No viable T. gondii was isolated from chicken hearts that were sampled. All seropositive (150) samples were from a total of 912 samples purchased from local markets.
Significance: The results suggest that T. gondii oocysts could present in the environment and infect the food animals. T. gondii prevalence in chicken hearts could reflect the environmental contamination of T. gondii and prevalence information can be used to manage T. gondii infection risk.