P1-105 Reveal 2.0 for Group D1 Salmonella Test for Raw Shell Eggs, Poultry Feed and Chicken Carcass Rinse

Monday, August 1, 2016
America's Center - St. Louis
Preetha Biswas, Neogen Corporation, Lansing, MI
Emily Feldpausch, Neogen Corporation, Lansing, MI
Lin Li, Neogen Corporation, Lansing, MI
Debra Foti, Neogen Corporation, Lansing, MI
Ryan Viator, Neogen Corporation, Lansing, MI
Quynh-Nhi Le, Neogen Corporation, Lansing, MI
Susan Alles, Neogen Corporation, Lansing, MI
Mark Mozola, Neogen Corporation, Lansing, MI
Jennifer Rice, Neogen Corporation, Lansing, MI
Introduction: Reveal 2.0 for Group D1 Salmonella is a lateral flow test device for detection of Salmonella Enteritidis (SE). The antibodies used in this device are broadly inclusive for SE and other serovars of the D1 somatic group. In 2014, this test was approved by the National Poultry Improvement Plan for use in testing poultry-house environmental samples.

Purpose: In this study, the Reveal test was evaluated for rapid detection of SE in raw shell eggs, poultry feed and chicken carcass rinse.

Methods: A low level of SE was inoculated into pooled raw shell eggs and a paired analysis was conducted utilizing a common primary enrichment method prescribed in the FDA/BAM procedure. Chicken carcass rinse and poultry feed samples spiked with SE were tested with the Reveal test and the USDA-FSIS/MLG or FDA/BAM reference methods, respectively. The novel secondary enrichment methodology incorporates modified semisolid Rappaport-Vassiliadis broth (MSRV) and the direct application of the Reveal device. For each matrix, 20 replicate test portions were inoculated to produce fractional positive levels, along with 5 high level and 5 uninoculated samples. Statistical analysis was conducted on the Reveal test and reference method data using probability of detection (POD) models.

Results: Method comparison data for fractional positive samples, at a 95% confidence interval, produced a dPOD of 0 (-0.14, +0.14) for raw shell egg samples, a dPOD of 0 (-0.26, +0.26) for chicken rinse samples and a dPOD of 0.3 (0, 0.54) for poultry feed samples. There was no statistically significant difference between performance of Reveal and reference methods. The Reveal test demonstrated inclusivity of 98% for target strains (49/50) and exclusivity of 100% for non-target strains (40/40) tested.

Significance: The study results demonstrate the utility of Reveal 2.0 for Group D1 Salmonella, as a rapid test for detection of SE in raw shell eggs, poultry feed and chicken carcass rinse.